Detailed information of TA system TA086972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1050..1693
• Replicon: plasmid p14EC033d
• Accession: NZ_CP024151
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CR539_RS25420
• Protein ID: WP_063073410.1
• Coordinates: 1277..1693 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A3Q0N5I0
• Locus tag: CR539_RS25415
• Protein ID: WP_063073411.1
• Coordinates: 1050..1280 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS25405	66..770	+	705	WP_001775011.1	IS6-like element IS26 family transposase	-
CR539_RS28310	914..1093	-	180	Protein_1	hypothetical protein	-
CR539_RS25415	1050..1280	+	231	WP_063073411.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CR539_RS25420	1277..1693	+	417	WP_063073410.1	type II toxin-antitoxin system VapC family toxin	Toxin
CR539_RS25425	1767..3329	+	1563	WP_063073409.1	AAA family ATPase	-
CR539_RS25430	3314..4336	+	1023	WP_063073408.1	hypothetical protein	-
CR539_RS25435	4623..4853	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CR539_RS25440	4850..4945	+	96	Protein_7	VapC toxin family PIN domain ribonuclease	-
CR539_RS25445	5004..5788	-	785	Protein_8	IS21-like element helper ATPase IstB	-
CR539_RS25450	5788..6441	-	654	Protein_9	IS21-like element IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..97858	97858
	-	inside	IScluster/Tn	-	-	66..6429	6363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15167.53 Da
• Isoelectric Point: 7.8882

>T86972 WP_063073410.1 NZ_CP024151:1277-1693 [Escherichia coli]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCTRLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHSIAAGAVLVTNNTREFERVPGLVLEDWIR

Nucleotide

• Length: 417 bp

>T86972 NZ_CP024151:1277-1693 [Escherichia coli]
GTGAACAAAACGTATATGCTGGACACCAACATCTGCTCTTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCCGAAATGCGCTTCGGGGCTACCGGCC
CGAAGGCCTCGCCGCGCCACGTGCAGCTGGTTGACGCGTTCTGCACCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGACGCGACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGACACTCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAACGGGTACCGGGTCTTGTGCTGG
AAGACTGGATAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8688.84 Da
• Isoelectric Point: 4.6310

>AT86972 WP_063073411.1 NZ_CP024151:1050-1280 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAKLEKADPDFMAEREDVVTDEGRVNL

Nucleotide

• Length: 231 bp

>AT86972 NZ_CP024151:1050-1280 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTTGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCGACCTGGGGCTCGTTCGCGAAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCACCGACGAAGGACGGGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q0N5I0