Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 79984..80543 Replicon plasmid p14EC033c
Accession NZ_CP024150
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name relE Uniprot ID A0A3Q0N4Y0
Locus tag CR539_RS25220 Protein ID WP_044789388.1
Coordinates 79984..80259 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A3Q0N511
Locus tag CR539_RS25225 Protein ID WP_044789390.1
Coordinates 80259..80543 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS25190 75687..76915 + 1229 WP_116315299.1 IS3 family transposase -
CR539_RS25195 76927..78066 - 1140 Protein_83 IS21 family transposase -
CR539_RS25200 78424..78678 + 255 Protein_84 IS3 family transposase -
CR539_RS25205 78689..78926 - 238 Protein_85 colicin V -
CR539_RS28125 78904..79140 - 237 WP_032257624.1 colicin V immunity protein -
CR539_RS25215 79603..79878 - 276 WP_044789386.1 hypothetical protein -
CR539_RS25220 79984..80259 - 276 WP_044789388.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR539_RS25225 80259..80543 - 285 WP_044789390.1 ribbon-helix-helix domain-containing protein Antitoxin
CR539_RS25240 81455..82312 - 858 WP_153071841.1 incFII family plasmid replication initiator RepA -
CR539_RS25245 82305..82379 - 75 WP_001375168.1 RepA leader peptide Tap -
CR539_RS25255 82615..82872 - 258 WP_000083826.1 replication regulatory protein RepA -
CR539_RS25260 83112..83702 - 591 WP_000766805.1 DUF2726 domain-containing protein -
CR539_RS25265 83740..83949 - 210 WP_001298565.1 hemolysin expression modulator Hha -
CR539_RS25270 83995..84456 - 462 WP_153071840.1 thermonuclease family protein -
CR539_RS25275 84701..84913 - 213 WP_001309245.1 ANR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..108710 108710


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10763.47 Da        Isoelectric Point: 9.9324

>T86971 WP_044789388.1 NZ_CP024150:c80259-79984 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREIRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T86971 NZ_CP024150:c80259-79984 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAGCCCAGGGAGATCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10446.88 Da        Isoelectric Point: 5.6558

>AT86971 WP_044789390.1 NZ_CP024150:c80543-80259 [Escherichia coli]
MQMKNNSAQATKVITAHVPLPMADKIDQMAAKLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT86971 NZ_CP024150:c80543-80259 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAATCGA
CCAGATGGCCGCCAAGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGATCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q0N4Y0

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q0N511

References