Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 27911..28376 Replicon plasmid p14EC033b
Accession NZ_CP024149
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag CR539_RS24675 Protein ID WP_001230707.1
Coordinates 27911..28162 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag CR539_RS24680 Protein ID WP_000801440.1
Coordinates 28149..28376 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS24650 23392..23919 - 528 WP_000577043.1 DUF2726 domain-containing protein -
CR539_RS24655 24054..24458 - 405 WP_012421203.1 hypothetical protein -
CR539_RS24660 24471..25337 - 867 WP_001353743.1 replication initiation protein -
CR539_RS28100 26752..27267 + 516 WP_001025395.1 J domain-containing protein -
CR539_RS24670 27372..27914 + 543 WP_001083906.1 hypothetical protein -
CR539_RS24675 27911..28162 + 252 WP_001230707.1 hypothetical protein Toxin
CR539_RS24680 28149..28376 + 228 WP_000801440.1 hypothetical protein Antitoxin
CR539_RS24690 28830..29129 + 300 WP_000650309.1 hypothetical protein -
CR539_RS24695 29220..29570 + 351 WP_000854259.1 hypothetical protein -
CR539_RS24700 29875..30123 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
CR539_RS24705 30120..30467 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
CR539_RS24715 30828..31331 + 504 WP_000781813.1 hypothetical protein -
CR539_RS24720 31335..32564 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
CR539_RS24725 32641..32934 + 294 WP_060598542.1 LuxR family transcriptional regulator -
CR539_RS24730 32931..33146 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33301 33301


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T86968 WP_001230707.1 NZ_CP024149:27911-28162 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T86968 NZ_CP024149:27911-28162 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT86968 WP_000801440.1 NZ_CP024149:28149-28376 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT86968 NZ_CP024149:28149-28376 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References