Detailed information of TA system TA086967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 56096..56597
• Replicon: plasmid p14EC033a
• Accession: NZ_CP024148
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: CR539_RS24435
• Protein ID: WP_000702247.1
• Coordinates: 56337..56597 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: CR539_RS24430
• Protein ID: WP_001132407.1
• Coordinates: 56096..56347 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS24380	51153..51905	+	753	WP_000797966.1	conjugal transfer protein TraL	-
CR539_RS24385	51907..52614	+	708	WP_021503228.1	hypothetical protein	-
CR539_RS24390	52634..52960	+	327	WP_063078684.1	hypothetical protein	-
CR539_RS24395	53091..53528	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
CR539_RS24400	53553..53735	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
CR539_RS24405	54293..54475	+	183	WP_001481829.1	hypothetical protein	-
CR539_RS24410	54582..54848	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
CR539_RS24415	55268..55546	-	279	WP_000545938.1	hypothetical protein	-
CR539_RS24430	56096..56347	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
CR539_RS24435	56337..56597	-	261	WP_000702247.1	hypothetical protein	Toxin
CR539_RS24440	56649..56873	-	225	WP_000814381.1	hypothetical protein	-
CR539_RS24445	56970..57179	-	210	WP_000866036.1	hypothetical protein	-
CR539_RS24450	57221..57544	-	324	WP_000427403.1	hypothetical protein	-
CR539_RS24460	58126..58455	-	330	WP_000855120.1	hypothetical protein	-
CR539_RS24470	59052..59717	+	666	WP_000532148.1	P-loop NTPase	-
CR539_RS28275	59791..60075	+	285	WP_001162371.1	hypothetical protein	-
CR539_RS24480	60093..60689	-	597	WP_000125165.1	hypothetical protein	-
CR539_RS24485	60839..61000	-	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..62585	62585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T86967 WP_000702247.1 NZ_CP024148:c56597-56337 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T86967 NZ_CP024148:c56597-56337 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT86967 WP_001132407.1 NZ_CP024148:c56347-56096 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT86967 NZ_CP024148:c56347-56096 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0