Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4391158..4391789 Replicon chromosome
Accession NZ_CP024147
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name hicA Uniprot ID A0A1M1UJK7
Locus tag CR539_RS22870 Protein ID WP_001259382.1
Coordinates 4391514..4391789 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CR539_RS22865 Protein ID WP_000593555.1
Coordinates 4391158..4391517 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS22840 4387290..4388234 + 945 WP_032281756.1 nickel ABC transporter permease subunit NikB -
CR539_RS22845 4388231..4389064 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CR539_RS22850 4389064..4389828 + 765 WP_032281757.1 nickel import ATP-binding protein NikD -
CR539_RS22855 4389825..4390631 + 807 WP_032288306.1 nickel import ATP-binding protein NikE -
CR539_RS22860 4390637..4391038 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CR539_RS22865 4391158..4391517 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CR539_RS22870 4391514..4391789 - 276 WP_001259382.1 type II toxin-antitoxin system HicA family toxin Toxin
CR539_RS22875 4391862..4392986 - 1125 WP_001314210.1 ABC transporter permease -
CR539_RS22880 4392986..4395721 - 2736 WP_032281760.1 ribosome-associated ATPase/putative transporter RbbA -
CR539_RS22885 4395718..4396785 - 1068 WP_032281761.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.91 Da        Isoelectric Point: 10.7076

>T86966 WP_001259382.1 NZ_CP024147:c4391789-4391514 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T86966 NZ_CP024147:c4391789-4391514 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGACAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT86966 WP_000593555.1 NZ_CP024147:c4391517-4391158 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT86966 NZ_CP024147:c4391517-4391158 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCTCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M1UJK7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References