Detailed information of TA system TA086964

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3959643..3960462
• Replicon: chromosome
• Accession: NZ_CP024147
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2I6F3V8
• Locus tag: CR539_RS20500
• Protein ID: WP_054627366.1
• Coordinates: 3959643..3960020 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CR539_RS20505
• Protein ID: WP_199560497.1
• Coordinates: 3960109..3960462 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS20470	3955273..3957114	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
CR539_RS20475	3957193..3957699	-	507	WP_032281621.1	G/U mismatch-specific DNA glycosylase	-
CR539_RS20485	3957999..3958844	-	846	WP_101969520.1	DUF4942 domain-containing protein	-
CR539_RS20490	3958941..3959138	-	198	WP_000772024.1	DUF957 domain-containing protein	-
CR539_RS20495	3959158..3959646	-	489	Protein_3702	hypothetical protein	-
CR539_RS20500	3959643..3960020	-	378	WP_054627366.1	type IV toxin-antitoxin system toxin CbtA	Toxin
CR539_RS20505	3960109..3960462	-	354	WP_199560497.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CR539_RS20510	3960542..3960763	-	222	WP_053265969.1	DUF987 domain-containing protein	-
CR539_RS20515	3960850..3961326	-	477	WP_158302277.1	RadC family protein	-
CR539_RS20520	3961341..3961826	-	486	WP_000214317.1	antirestriction protein	-
CR539_RS20525	3961917..3962735	-	819	WP_001234702.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13928.97 Da
• Isoelectric Point: 8.5163

>T86964 WP_054627366.1 NZ_CP024147:c3960020-3959643 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T86964 NZ_CP024147:c3960020-3959643 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13223.15 Da
• Isoelectric Point: 7.7473

>AT86964 WP_199560497.1 NZ_CP024147:c3960462-3960109 [Escherichia coli]
VSDTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPR
HQHTVTLYARGLTCKADTLSSCGYVYLAVYPMPKMKN

Nucleotide

• Length: 354 bp

>AT86964 NZ_CP024147:c3960462-3960109 [Escherichia coli]
GTGTCAGACACACTCCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACAGTGACGCCCTGTTTCGGGGC
ACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGGCGGTTCAGCAATGCAGATG
CGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGTGAACTGAATCCCCGC
CATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTGTGGTTACGTTTATCT
GGCTGTTTATCCGATGCCCAAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A2I6F3V8