Detailed information of TA system TA086951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2265597..2266235
• Replicon: chromosome
• Accession: NZ_CP024147
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A080J2T0
• Locus tag: CR539_RS11735
• Protein ID: WP_000813793.1
• Coordinates: 2265597..2265773 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CR539_RS11740
• Protein ID: WP_077757373.1
• Coordinates: 2265819..2266235 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS11715	2261213..2262430	-	1218	WP_099145411.1	BenE family transporter YdcO	-
CR539_RS11720	2262483..2263019	+	537	WP_000429152.1	DNA-binding transcriptional regulator SutR	-
CR539_RS11725	2263092..2265053	+	1962	WP_032280834.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CR539_RS11730	2265145..2265375	-	231	WP_000494244.1	YncJ family protein	-
CR539_RS11735	2265597..2265773	+	177	WP_000813793.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CR539_RS11740	2265819..2266235	+	417	WP_077757373.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CR539_RS11745	2266314..2267720	+	1407	WP_032280836.1	PLP-dependent aminotransferase family protein	-
CR539_RS11750	2267965..2269110	+	1146	WP_032280837.1	ABC transporter substrate-binding protein	-
CR539_RS11755	2269128..2270141	+	1014	WP_032288192.1	ABC transporter ATP-binding protein	-
CR539_RS11760	2270142..2271083	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-67)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.83 Da
• Isoelectric Point: 10.9223

>T86951 WP_000813793.1 NZ_CP024147:2265597-2265773 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGMRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T86951 NZ_CP024147:2265597-2265773 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGATGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15228.60 Da
• Isoelectric Point: 4.7386

>AT86951 WP_077757373.1 NZ_CP024147:2265819-2266235 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVLV

Nucleotide

• Length: 417 bp

>AT86951 NZ_CP024147:2265819-2266235 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGTCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGCTGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A080J2T0