Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1270962..1271580 Replicon chromosome
Accession NZ_CP024147
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CR539_RS06585 Protein ID WP_001291435.1
Coordinates 1270962..1271180 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CR539_RS06590 Protein ID WP_000344800.1
Coordinates 1271206..1271580 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS06540 1266076..1266387 - 312 WP_000409911.1 MGMT family protein -
CR539_RS06550 1266766..1267119 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CR539_RS06555 1267161..1268216 - 1056 Protein_1172 cyclic-guanylate-specific phosphodiesterase PdeB -
CR539_RS06565 1268272..1268969 + 698 Protein_1173 IS1 family transposase -
CR539_RS06570 1268983..1269489 - 507 Protein_1174 cyclic-guanylate-specific phosphodiesterase PdeB -
CR539_RS06575 1269653..1270123 - 471 WP_000136192.1 YlaC family protein -
CR539_RS06580 1270239..1270790 - 552 WP_000102564.1 maltose O-acetyltransferase -
CR539_RS06585 1270962..1271180 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CR539_RS06590 1271206..1271580 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CR539_RS06595 1272126..1275275 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CR539_RS06600 1275298..1276491 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T86949 WP_001291435.1 NZ_CP024147:c1271180-1270962 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T86949 NZ_CP024147:c1271180-1270962 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT86949 WP_000344800.1 NZ_CP024147:c1271580-1271206 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT86949 NZ_CP024147:c1271580-1271206 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References