Detailed information of TA system TA086948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1235875..1236712
• Replicon: chromosome
• Accession: NZ_CP024147
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A080JA52
• Locus tag: CR539_RS06390
• Protein ID: WP_032170811.1
• Coordinates: 1235875..1236417 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A080JFU1
• Locus tag: CR539_RS06395
• Protein ID: WP_001577859.1
• Coordinates: 1236401..1236712 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS06370	1231415..1232326	-	912	WP_000705869.1	2-dehydropantoate 2-reductase	-
CR539_RS06375	1232494..1232985	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
CR539_RS06380	1233113..1234477	-	1365	WP_001000956.1	MFS transporter	-
CR539_RS06385	1234884..1235819	+	936	WP_032280441.1	sel1 repeat family protein	-
CR539_RS06390	1235875..1236417	-	543	WP_032170811.1	GNAT family N-acetyltransferase	Toxin
CR539_RS06395	1236401..1236712	-	312	WP_001577859.1	DUF1778 domain-containing protein	Antitoxin
CR539_RS06400	1236897..1237787	-	891	WP_000971336.1	heme o synthase	-
CR539_RS06405	1237799..1238128	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CR539_RS06410	1238128..1238742	-	615	WP_032280399.1	cytochrome o ubiquinol oxidase subunit III	-
CR539_RS06415	1238732..1240723	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CR539_RS06420	1240745..1241692	-	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	espY3	1223271..1236712	13441

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.05 Da
• Isoelectric Point: 8.3395

>T86948 WP_032170811.1 NZ_CP024147:c1236417-1235875 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRTSLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T86948 NZ_CP024147:c1236417-1235875 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCACATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAGCCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTGGGGGTGGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11400.08 Da
• Isoelectric Point: 10.5548

>AT86948 WP_001577859.1 NZ_CP024147:c1236712-1236401 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNRLREAAQAVGLDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT86948 NZ_CP024147:c1236712-1236401 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAGACTTCGTGAAGCTGCGCAAGCCGTTGGGTTAGATTTAAGCGCCTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGCGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCC
GAACCTGCTCAACCGACGCTCGCACTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A080JA52

Antitoxin

• AlphaFold DB: A0A080JFU1