Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 832413..832963 Replicon chromosome
Accession NZ_CP024147
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag CR539_RS04280 Protein ID WP_001160974.1
Coordinates 832649..832963 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CR539_RS04275 Protein ID WP_000125566.1
Coordinates 832413..832646 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS04250 827484..827771 + 288 WP_000203741.1 ferredoxin-like protein FixX -
CR539_RS04255 827829..829160 + 1332 WP_032280232.1 MFS transporter -
CR539_RS04260 829271..829801 + 531 WP_000600737.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CR539_RS04265 829794..831656 + 1863 WP_000377186.1 glutathione-regulated potassium-efflux system protein KefC -
CR539_RS04270 831848..832327 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
CR539_RS04275 832413..832646 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CR539_RS04280 832649..832963 + 315 WP_001160974.1 CcdB family protein Toxin
CR539_RS04285 832960..833808 - 849 WP_000257188.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CR539_RS04290 833815..834192 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CR539_RS04295 834195..835016 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CR539_RS04300 835013..836002 - 990 WP_032280236.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CR539_RS04305 836002..837288 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T86945 WP_001160974.1 NZ_CP024147:832649-832963 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T86945 NZ_CP024147:832649-832963 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT86945 WP_000125566.1 NZ_CP024147:832413-832646 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT86945 NZ_CP024147:832413-832646 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References