Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 686077..686780 Replicon chromosome
Accession NZ_CP024147
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name ypjF Uniprot ID A0A3P1KY50
Locus tag CR539_RS03475 Protein ID WP_001757895.1
Coordinates 686439..686780 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A3K4FCA5
Locus tag CR539_RS03470 Protein ID WP_024185730.1
Coordinates 686077..686418 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS03435 681230..682105 + 876 WP_001757886.1 GTPase family protein -
CR539_RS03440 682349..683065 + 717 WP_101969307.1 WYL domain-containing protein -
CR539_RS03445 683101..683553 + 453 WP_077264947.1 hypothetical protein -
CR539_RS03450 683625..684098 + 474 WP_024185729.1 hypothetical protein -
CR539_RS03455 684219..685040 + 822 WP_001757890.1 DUF945 domain-containing protein -
CR539_RS03460 685071..685511 + 441 WP_001757892.1 antirestriction protein -
CR539_RS03465 685524..686066 + 543 WP_001757893.1 DNA repair protein RadC -
CR539_RS03470 686077..686418 + 342 WP_024185730.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CR539_RS03475 686439..686780 + 342 WP_001757895.1 TA system toxin CbtA family protein Toxin
CR539_RS03480 687044..688051 + 1008 WP_001757896.1 restriction endonuclease -
CR539_RS03500 689015..689665 + 651 WP_001037966.1 HNH endonuclease -
CR539_RS03505 689673..690653 - 981 WP_001295601.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE 655402..695689 40287


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-109)

Antitoxin

Antitoxin CbeA

(13-111)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12759.74 Da        Isoelectric Point: 9.6548

>T86943 WP_001757895.1 NZ_CP024147:686439-686780 [Escherichia coli]
MKTLPATNPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQQHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Download         Length: 342 bp

>T86943 NZ_CP024147:686439-686780 [Escherichia coli]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGCAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12744.41 Da        Isoelectric Point: 6.7345

>AT86943 WP_024185730.1 NZ_CP024147:686077-686418 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDMAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Download         Length: 342 bp

>AT86943 NZ_CP024147:686077-686418 [Escherichia coli]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATATGGCATTCCCGCATTTTATTAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P1KY50

Antitoxin

Source ID Structure
AlphaFold DB A0A3K4FCA5

References