Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 7607..8142 Replicon plasmid p14EC029d
Accession NZ_CP024145
Organism Escherichia coli strain 14EC029

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag CR538_RS28380 Protein ID WP_001270417.1
Coordinates 7607..7894 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag CR538_RS28385 Protein ID WP_001132895.1
Coordinates 7891..8142 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR538_RS28335 3158..3267 + 110 Protein_3 IS5/IS1182 family transposase -
CR538_RS28340 3790..4040 + 251 Protein_4 type II toxin-antitoxin system ParD family antitoxin -
CR538_RS28360 4678..5418 + 741 WP_101968912.1 tyrosine-type recombinase/integrase -
CR538_RS28365 5703..6680 - 978 WP_000361610.1 RepB family plasmid replication initiator protein -
CR538_RS28375 7360..7608 - 249 Protein_7 integrase core domain-containing protein -
CR538_RS28380 7607..7894 - 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR538_RS28385 7891..8142 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CR538_RS28390 8315..8527 - 213 WP_000402944.1 hypothetical protein -
CR538_RS28400 8901..10106 + 1206 WP_000817634.1 AAA family ATPase -
CR538_RS28405 10103..11065 + 963 WP_000756327.1 ParB/RepB/Spo0J family partition protein -
CR538_RS28410 11299..12076 + 778 Protein_13 hypothetical protein -
CR538_RS29885 12243..12386 + 144 WP_000270807.1 DUF1281 domain-containing protein -
CR538_RS29890 12393..12752 + 360 WP_001385002.1 DUF1281 domain-containing protein -
CR538_RS28430 12816..13127 + 312 WP_001309256.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - eltB / eltA 1..106478 106478


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T86936 WP_001270417.1 NZ_CP024145:c7894-7607 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T86936 NZ_CP024145:c7894-7607 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGTGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT86936 WP_001132895.1 NZ_CP024145:c8142-7891 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT86936 NZ_CP024145:c8142-7891 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References