Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 48555..49198 Replicon plasmid p14EC029c
Accession NZ_CP024144
Organism Escherichia coli strain 14EC029

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag CR538_RS28000 Protein ID WP_001044768.1
Coordinates 48782..49198 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag CR538_RS27995 Protein ID WP_001261287.1
Coordinates 48555..48785 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR538_RS27955 43840..45534 - 1695 WP_000105636.1 mercury(II) reductase -
CR538_RS27960 45586..46008 - 423 WP_001340589.1 organomercurial transporter MerC -
CR538_RS27965 46044..46319 - 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
CR538_RS27970 46333..46683 - 351 WP_001294663.1 mercuric transport protein MerT -
CR538_RS27975 46755..47189 + 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
CR538_RS27980 47236..47940 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
CR538_RS27985 47987..48259 - 273 WP_061602529.1 hypothetical protein -
CR538_RS27995 48555..48785 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CR538_RS28000 48782..49198 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
CR538_RS28005 49360..51497 - 2138 Protein_55 AAA family ATPase -
CR538_RS28010 51851..52108 + 258 WP_000343085.1 hypothetical protein -
CR538_RS28015 52108..52698 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B) / aph(3')-Ia - 1..96973 96973
- flank IS/Tn - - 47236..47940 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T86935 WP_001044768.1 NZ_CP024144:48782-49198 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T86935 NZ_CP024144:48782-49198 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT86935 WP_001261287.1 NZ_CP024144:48555-48785 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT86935 NZ_CP024144:48555-48785 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References