Detailed information of TA system TA086933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 57417..57939
• Replicon: plasmid p14EC029a
• Accession: NZ_CP024142
• Organism: Escherichia coli strain 14EC029

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: CR538_RS26190
• Protein ID: WP_000638823.1
• Coordinates: 57417..57698 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: CR538_RS26195
• Protein ID: WP_000121741.1
• Coordinates: 57688..57939 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR538_RS26145	52494..52814	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
CR538_RS26150	52885..53073	-	189	WP_050567133.1	hypothetical protein	-
CR538_RS26155	53175..53759	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
CR538_RS26160	53780..54178	-	399	WP_001153665.1	hypothetical protein	-
CR538_RS26165	54297..54734	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
CR538_RS26170	54740..55975	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
CR538_RS26175	55978..56112	-	135	Protein_58	hypothetical protein	-
CR538_RS26180	56123..57094	-	972	WP_170386723.1	IS30-like element ISApl1 family transposase	-
CR538_RS26185	57185..57349	-	165	Protein_60	conjugal transfer protein	-
CR538_RS26190	57417..57698	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR538_RS26195	57688..57939	-	252	WP_000121741.1	hypothetical protein	Antitoxin
CR538_RS26200	58039..58674	-	636	WP_015059536.1	hypothetical protein	-
CR538_RS26205	58818..59531	+	714	WP_101968904.1	DNA primase	-
CR538_RS26210	59754..59978	-	225	WP_000713562.1	EexN family lipoprotein	-
CR538_RS26215	59987..60631	-	645	WP_001542014.1	hypothetical protein	-
CR538_RS26220	60637..61632	-	996	WP_001028540.1	type IV secretion system protein	-
CR538_RS26225	61636..61893	-	258	WP_000739143.1	hypothetical protein	-
CR538_RS26230	61890..62192	-	303	WP_001360345.1	hypothetical protein	-
CR538_RS26235	62173..62430	-	258	WP_000285945.1	hypothetical protein	-
CR538_RS26240	62463..62909	-	447	WP_001243164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..66596	66596
	-	flank	IS/Tn	-	-	56123..57046	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T86933 WP_000638823.1 NZ_CP024142:c57698-57417 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T86933 NZ_CP024142:c57698-57417 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT86933 WP_000121741.1 NZ_CP024142:c57939-57688 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT86933 NZ_CP024142:c57939-57688 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1