Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 5239..5732 Replicon plasmid p14EC029a
Accession NZ_CP024142
Organism Escherichia coli strain 14EC029

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag CR538_RS25885 Protein ID WP_001230709.1
Coordinates 5478..5732 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag CR538_RS25880 Protein ID WP_000151456.1
Coordinates 5239..5481 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR538_RS25855 2119..2523 - 405 WP_199562948.1 IS200/IS605 family transposase -
CR538_RS25860 2582..3808 + 1227 WP_001672025.1 transposase -
CR538_RS25870 4375..4668 - 294 WP_000673428.1 hypothetical protein -
CR538_RS25880 5239..5481 - 243 WP_000151456.1 hypothetical protein Antitoxin
CR538_RS25885 5478..5732 - 255 WP_001230709.1 hypothetical protein Toxin
CR538_RS25890 5729..6271 - 543 WP_001083904.1 hypothetical protein -
CR538_RS25895 6375..6890 - 516 WP_001025393.1 J domain-containing protein -
CR538_RS25900 7294..7623 - 330 WP_000855119.1 hypothetical protein -
CR538_RS25910 8211..8876 + 666 WP_000532148.1 P-loop NTPase -
CR538_RS30060 8950..9234 + 285 WP_001162371.1 hypothetical protein -
CR538_RS25920 9252..9848 - 597 WP_000125165.1 hypothetical protein -
CR538_RS25925 9998..10159 - 162 WP_000775236.1 type I toxin-antitoxin system Hok family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..66596 66596


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-88)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T86932 WP_001230709.1 NZ_CP024142:c5732-5478 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T86932 NZ_CP024142:c5732-5478 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT86932 WP_000151456.1 NZ_CP024142:c5481-5239 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT86932 NZ_CP024142:c5481-5239 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References