Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3667940..3668558 Replicon chromosome
Accession NZ_CP024141
Organism Escherichia coli strain 14EC029

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CR538_RS19205 Protein ID WP_001291435.1
Coordinates 3668340..3668558 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CR538_RS19200 Protein ID WP_000344800.1
Coordinates 3667940..3668314 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR538_RS19190 3663029..3664222 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CR538_RS19195 3664245..3667394 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CR538_RS19200 3667940..3668314 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CR538_RS19205 3668340..3668558 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CR538_RS19210 3668730..3669281 + 552 WP_000102558.1 maltose O-acetyltransferase -
CR538_RS19215 3669397..3669867 + 471 WP_000136192.1 YlaC family protein -
CR538_RS19220 3670031..3671581 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CR538_RS19225 3671623..3671976 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CR538_RS19235 3672355..3672666 + 312 WP_000409911.1 MGMT family protein -
CR538_RS19240 3672697..3673269 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T86928 WP_001291435.1 NZ_CP024141:3668340-3668558 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T86928 NZ_CP024141:3668340-3668558 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT86928 WP_000344800.1 NZ_CP024141:3667940-3668314 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT86928 NZ_CP024141:3667940-3668314 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References