Detailed information of TA system TA086927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2411903..2412429
• Replicon: chromosome
• Accession: NZ_CP024141
• Organism: Escherichia coli strain 14EC029

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CR538_RS12700
• Protein ID: WP_000323025.1
• Coordinates: 2412142..2412429 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CR538_RS12695
• Protein ID: WP_000534858.1
• Coordinates: 2411903..2412142 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR538_RS12660	2407265..2407621	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
CR538_RS29955	2408232..2408519	+	288	Protein_2309	hypothetical protein	-
CR538_RS12675	2409288..2410601	+	1314	WP_000589005.1	ISNCY family transposase	-
CR538_RS12685	2411038..2411370	-	333	WP_001301033.1	protein FlxA	-
CR538_RS12690	2411573..2411878	-	306	WP_001326990.1	hypothetical protein	-
CR538_RS12695	2411903..2412142	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CR538_RS12700	2412142..2412429	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CR538_RS12705	2412501..2412656	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CR538_RS12710	2412873..2413124	+	252	WP_000980994.1	hypothetical protein	-
CR538_RS12715	2413191..2413469	+	279	WP_012304870.1	hypothetical protein	-
CR538_RS12720	2413471..2414520	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
CR538_RS12725	2414534..2415286	+	753	WP_001047135.1	antitermination protein	-
CR538_RS29695	2415564..2415653	-	90	WP_158298097.1	hypothetical protein	-
CR538_RS12730	2415708..2415920	-	213	WP_000087756.1	cold shock-like protein CspF	-
CR538_RS12735	2416221..2416436	+	216	WP_000066484.1	cold shock-like protein CspB	-
CR538_RS30235	2416800..2416970	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
CR538_RS12740	2417190..2417405	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2387031..2449531	62500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T86927 WP_000323025.1 NZ_CP024141:2412142-2412429 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T86927 NZ_CP024141:2412142-2412429 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT86927 WP_000534858.1 NZ_CP024141:2411903-2412142 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT86927 NZ_CP024141:2411903-2412142 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8