Detailed information of TA system TA086917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1393329..1393954
• Replicon: chromosome
• Accession: NZ_CP024141
• Organism: Escherichia coli strain 14EC029

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CR538_RS07360
• Protein ID: WP_000911330.1
• Coordinates: 1393556..1393954 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CR538_RS07355
• Protein ID: WP_000450524.1
• Coordinates: 1393329..1393556 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR538_RS07330	1389132..1389602	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CR538_RS07335	1389602..1390174	-	573	WP_101968743.1	glycine cleavage system transcriptional repressor	-
CR538_RS07340	1390320..1391198	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CR538_RS07345	1391215..1392249	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CR538_RS07350	1392462..1393175	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CR538_RS07355	1393329..1393556	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CR538_RS07360	1393556..1393954	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CR538_RS07365	1394101..1394964	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
CR538_RS07370	1394979..1396994	+	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
CR538_RS07375	1397068..1397766	+	699	WP_000679812.1	esterase	-
CR538_RS07380	1397876..1398076	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T86917 WP_000911330.1 NZ_CP024141:1393556-1393954 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T86917 NZ_CP024141:1393556-1393954 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT86917 WP_000450524.1 NZ_CP024141:1393329-1393556 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT86917 NZ_CP024141:1393329-1393556 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33