Detailed information of TA system TA086912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 322538..323338
• Replicon: chromosome
• Accession: NZ_CP024141
• Organism: Escherichia coli strain 14EC029

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: CR538_RS01655
• Protein ID: WP_000342449.1
• Coordinates: 322811..323338 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: CR538_RS01650
• Protein ID: WP_001277108.1
• Coordinates: 322538..322804 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR538_RS01625	318195..318863	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
CR538_RS01630	318856..319914	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
CR538_RS01635	320159..321013	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
CR538_RS01645	321285..322388	+	1104	WP_001350438.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
CR538_RS01650	322538..322804	+	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
CR538_RS01655	322811..323338	+	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
CR538_RS01660	323335..323718	-	384	WP_000778781.1	aspartate 1-decarboxylase autocleavage activator PanM	-
CR538_RS01670	324142..325251	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
CR538_RS01675	325299..326225	+	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
CR538_RS01680	326222..327499	+	1278	WP_000803799.1	branched chain amino acid ABC transporter permease LivM	-
CR538_RS01685	327496..328263	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T86912 WP_000342449.1 NZ_CP024141:322811-323338 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T86912 NZ_CP024141:322811-323338 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT86912 WP_001277108.1 NZ_CP024141:322538-322804 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT86912 NZ_CP024141:322538-322804 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24