Detailed information of TA system TA086900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 51016..51538
• Replicon: plasmid p14EC020a
• Accession: NZ_CP024139
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: CR537_RS25760
• Protein ID: WP_000638823.1
• Coordinates: 51016..51297 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: CR537_RS25765
• Protein ID: WP_000121741.1
• Coordinates: 51287..51538 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS25725	47165..47485	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
CR537_RS25730	47556..47744	-	189	WP_050567133.1	hypothetical protein	-
CR537_RS25735	47846..48430	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
CR537_RS25740	48451..48849	-	399	WP_001153665.1	hypothetical protein	-
CR537_RS25745	48968..49405	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
CR537_RS25750	49411..50646	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
CR537_RS25755	50649..50948	-	300	WP_000835764.1	IncN plasmid KikA protein	-
CR537_RS25760	51016..51297	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR537_RS25765	51287..51538	-	252	WP_000121741.1	hypothetical protein	Antitoxin
CR537_RS25770	51638..52273	-	636	WP_015059536.1	hypothetical protein	-
CR537_RS25775	52285..53130	+	846	WP_199561150.1	DNA primase	-
CR537_RS25780	53353..53577	-	225	WP_065304445.1	EexN family lipoprotein	-
CR537_RS25785	53586..54230	-	645	WP_065304453.1	conjugal transfer protein TrbJ	-
CR537_RS25790	54236..55231	-	996	WP_065304446.1	type IV secretion system protein	-
CR537_RS25795	55235..55492	-	258	WP_000739144.1	hypothetical protein	-
CR537_RS25800	55489..55791	-	303	WP_001360345.1	hypothetical protein	-
CR537_RS25805	55772..56029	-	258	WP_001542015.1	hypothetical protein	-
CR537_RS25810	56062..56508	-	447	WP_001243165.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..64765	64765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T86900 WP_000638823.1 NZ_CP024139:c51297-51016 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T86900 NZ_CP024139:c51297-51016 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT86900 WP_000121741.1 NZ_CP024139:c51538-51287 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT86900 NZ_CP024139:c51538-51287 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1