Detailed information of TA system TA086899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 7914..8506
• Replicon: plasmid p14EC020a
• Accession: NZ_CP024139
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: CR537_RS25520
• Protein ID: WP_000513532.1
• Coordinates: 7914..8162 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: CR537_RS25525
• Protein ID: WP_000681609.1
• Coordinates: 8159..8506 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS25475	2926..3591	-	666	WP_000532148.1	P-loop NTPase	-
CR537_RS25485	4180..4509	+	330	WP_001542022.1	hypothetical protein	-
CR537_RS25490	4910..5425	+	516	WP_001025393.1	J domain-containing protein	-
CR537_RS25495	5529..6071	+	543	WP_001083904.1	hypothetical protein	-
CR537_RS25500	6068..6322	+	255	WP_001230709.1	hypothetical protein	-
CR537_RS25505	6319..6561	+	243	WP_000151456.1	hypothetical protein	-
CR537_RS25515	7132..7425	+	294	WP_000673428.1	hypothetical protein	-
CR537_RS25520	7914..8162	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
CR537_RS25525	8159..8506	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CR537_RS25530	8594..8776	-	183	WP_001428470.1	hypothetical protein	-
CR537_RS25535	9278..9577	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
CR537_RS25540	9837..10163	-	327	WP_000206697.1	hypothetical protein	-
CR537_RS25545	10165..10605	-	441	WP_023223352.1	hypothetical protein	-
CR537_RS25550	10625..11332	-	708	WP_000108794.1	hypothetical protein	-
CR537_RS25555	11334..12086	-	753	WP_000797966.1	conjugal transfer protein TraL	-
CR537_RS25560	12104..12505	-	402	WP_021575837.1	hypothetical protein	-
CR537_RS25565	12874..13215	+	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..64765	64765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T86899 WP_000513532.1 NZ_CP024139:7914-8162 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T86899 NZ_CP024139:7914-8162 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT86899 WP_000681609.1 NZ_CP024139:8159-8506 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT86899 NZ_CP024139:8159-8506 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49