Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6068..6561 Replicon plasmid p14EC020a
Accession NZ_CP024139
Organism Escherichia coli strain 14EC020

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag CR537_RS25500 Protein ID WP_001230709.1
Coordinates 6068..6322 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag CR537_RS25505 Protein ID WP_000151456.1
Coordinates 6319..6561 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR537_RS25460 1643..1804 + 162 WP_000775236.1 type I toxin-antitoxin system Hok family toxin -
CR537_RS25465 1954..2550 + 597 WP_000125165.1 hypothetical protein -
CR537_RS27345 2568..2852 - 285 WP_001162371.1 hypothetical protein -
CR537_RS25475 2926..3591 - 666 WP_000532148.1 P-loop NTPase -
CR537_RS25485 4180..4509 + 330 WP_001542022.1 hypothetical protein -
CR537_RS25490 4910..5425 + 516 WP_001025393.1 J domain-containing protein -
CR537_RS25495 5529..6071 + 543 WP_001083904.1 hypothetical protein -
CR537_RS25500 6068..6322 + 255 WP_001230709.1 hypothetical protein Toxin
CR537_RS25505 6319..6561 + 243 WP_000151456.1 hypothetical protein Antitoxin
CR537_RS25515 7132..7425 + 294 WP_000673428.1 hypothetical protein -
CR537_RS25520 7914..8162 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
CR537_RS25525 8159..8506 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
CR537_RS25530 8594..8776 - 183 WP_001428470.1 hypothetical protein -
CR537_RS25535 9278..9577 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
CR537_RS25540 9837..10163 - 327 WP_000206697.1 hypothetical protein -
CR537_RS25545 10165..10605 - 441 WP_023223352.1 hypothetical protein -
CR537_RS25550 10625..11332 - 708 WP_000108794.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..64765 64765


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-69)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T86898 WP_001230709.1 NZ_CP024139:6068-6322 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T86898 NZ_CP024139:6068-6322 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT86898 WP_000151456.1 NZ_CP024139:6319-6561 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT86898 NZ_CP024139:6319-6561 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References