Detailed information of TA system TA086888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2590566..2591202
• Replicon: chromosome
• Accession: NZ_CP024138
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: CR537_RS13365
• Protein ID: WP_000813796.1
• Coordinates: 2591026..2591202 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CR537_RS13360
• Protein ID: WP_076838470.1
• Coordinates: 2590566..2590982 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS13340	2585718..2586659	-	942	WP_001251307.1	ABC transporter permease	-
CR537_RS13345	2586660..2587673	-	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
CR537_RS13350	2587691..2588836	-	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
CR537_RS13355	2589081..2590487	-	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
CR537_RS13360	2590566..2590982	-	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CR537_RS13365	2591026..2591202	-	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CR537_RS13370	2591424..2591654	+	231	WP_000494243.1	YncJ family protein	-
CR537_RS13375	2591746..2593707	-	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CR537_RS13380	2593780..2594316	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
CR537_RS13385	2594369..2595580	+	1212	WP_071593090.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T86888 WP_000813796.1 NZ_CP024138:c2591202-2591026 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T86888 NZ_CP024138:c2591202-2591026 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT86888 WP_076838470.1 NZ_CP024138:c2590982-2590566 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT86888 NZ_CP024138:c2590982-2590566 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3