Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1389828..1390453 Replicon chromosome
Accession NZ_CP024138
Organism Escherichia coli strain 14EC020

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CR537_RS07055 Protein ID WP_000911329.1
Coordinates 1390055..1390453 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CR537_RS07050 Protein ID WP_000450524.1
Coordinates 1389828..1390055 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR537_RS07025 1385628..1386098 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
CR537_RS07030 1386098..1386670 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CR537_RS07035 1386816..1387694 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
CR537_RS07040 1387711..1388745 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CR537_RS07045 1388958..1389671 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CR537_RS07050 1389828..1390055 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CR537_RS07055 1390055..1390453 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
CR537_RS07060 1390600..1391463 + 864 WP_001267498.1 neutral zinc metallopeptidase -
CR537_RS07065 1391478..1393493 + 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
CR537_RS07070 1393567..1394265 + 699 WP_000679819.1 esterase -
CR537_RS07075 1394346..1394546 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T86874 WP_000911329.1 NZ_CP024138:1390055-1390453 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T86874 NZ_CP024138:1390055-1390453 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT86874 WP_000450524.1 NZ_CP024138:1389828-1390055 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT86874 NZ_CP024138:1389828-1390055 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References