Detailed information of TA system TA086870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 354019..354650
• Replicon: chromosome
• Accession: NZ_CP024138
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: CR537_RS01800
• Protein ID: WP_001260301.1
• Coordinates: 354019..354294 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: CR537_RS01805
• Protein ID: WP_000593557.1
• Coordinates: 354291..354650 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS01785	349023..350090	+	1068	WP_000361470.1	HlyD family secretion protein	-
CR537_RS01790	350087..352822	+	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
CR537_RS01795	352822..353946	+	1125	WP_001318088.1	ABC transporter permease	-
CR537_RS01800	354019..354294	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
CR537_RS01805	354291..354650	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CR537_RS01810	354770..355171	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
CR537_RS01815	355177..355983	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
CR537_RS01820	355980..356744	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
CR537_RS01825	356744..357577	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
CR537_RS01830	357574..358518	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T86870 WP_001260301.1 NZ_CP024138:354019-354294 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T86870 NZ_CP024138:354019-354294 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT86870 WP_000593557.1 NZ_CP024138:354291-354650 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT86870 NZ_CP024138:354291-354650 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1