Detailed information of TA system TA086863

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 44836..45671
• Replicon: chromosome
• Accession: NZ_CP024138
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TIK8
• Locus tag: CR537_RS00260
• Protein ID: WP_000854694.1
• Coordinates: 44836..45213 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8S7S1S5
• Locus tag: CR537_RS00265
• Protein ID: WP_065793167.1
• Coordinates: 45303..45671 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS00220	39970..40263	-	294	WP_001297978.1	hypothetical protein	-
CR537_RS00225	40485..41303	+	819	WP_000779405.1	lipoprotein NlpA	-
CR537_RS00230	41307..42230	-	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
CR537_RS00235	42397..42894	-	498	WP_000509808.1	hypothetical protein	-
CR537_RS00245	43490..44332	-	843	WP_001696593.1	DUF4942 domain-containing protein	-
CR537_RS00250	44417..44614	-	198	WP_086795284.1	DUF957 domain-containing protein	-
CR537_RS00255	44690..44839	-	150	Protein_45	hypothetical protein	-
CR537_RS00260	44836..45213	-	378	WP_000854694.1	TA system toxin CbtA family protein	Toxin
CR537_RS00265	45303..45671	-	369	WP_065793167.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
CR537_RS00270	45834..46055	-	222	WP_000692303.1	DUF987 domain-containing protein	-
CR537_RS00275	46118..46594	-	477	WP_001186710.1	RadC family protein	-
CR537_RS00280	46606..47085	-	480	WP_000860065.1	antirestriction protein	-
CR537_RS00285	47167..47985	-	819	WP_001175149.1	DUF945 domain-containing protein	-
CR537_RS00290	48075..48308	-	234	WP_001278287.1	DUF905 family protein	-
CR537_RS00295	48314..48991	-	678	WP_001097305.1	hypothetical protein	-
CR537_RS00300	49139..49818	-	680	Protein_54	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14008.02 Da
• Isoelectric Point: 7.7293

>T86863 WP_000854694.1 NZ_CP024138:c45213-44836 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T86863 NZ_CP024138:c45213-44836 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13555.50 Da
• Isoelectric Point: 6.6249

>AT86863 WP_065793167.1 NZ_CP024138:c45671-45303 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT86863 NZ_CP024138:c45671-45303 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2F934