Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 14898..15433 Replicon plasmid p14EC017c
Accession NZ_CP024137
Organism Escherichia coli strain 14EC017

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag CR536_RS28775 Protein ID WP_000222766.1
Coordinates 14898..15185 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag CR536_RS28780 Protein ID WP_001132900.1
Coordinates 15182..15433 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR536_RS28760 11736..12949 + 1214 WP_162841971.1 IS3 family transposase -
CR536_RS28770 13403..14616 - 1214 Protein_11 IS3 family transposase -
CR536_RS28775 14898..15185 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR536_RS28780 15182..15433 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CR536_RS28800 16364..17577 + 1214 WP_162829241.1 IS3 family transposase -
CR536_RS28810 18111..18326 - 216 Protein_15 IS3 family transposase -
CR536_RS28815 18401..18802 - 402 WP_001222320.1 GspS family T2SS pilot lipoprotein variant EptO -
CR536_RS28820 18896..19729 - 834 WP_000088802.1 A24 family peptidase -
CR536_RS28825 19781..20296 - 516 WP_000998852.1 type II secretion system protein M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD exeG / exeE / stcE / nleA/espI 1..107279 107279
- inside IScluster/Tn - - 3422..17577 14155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T86862 WP_000222766.1 NZ_CP024137:c15185-14898 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T86862 NZ_CP024137:c15185-14898 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT86862 WP_001132900.1 NZ_CP024137:c15433-15182 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT86862 NZ_CP024137:c15433-15182 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References