Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 78050..78572 Replicon plasmid p14EC017b
Accession NZ_CP024136
Organism Escherichia coli strain 14EC017

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag CR536_RS28580 Protein ID WP_000220561.1
Coordinates 78050..78331 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag CR536_RS28585 Protein ID WP_000121743.1
Coordinates 78321..78572 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR536_RS28550 73527..73763 - 237 Protein_74 hypothetical protein -
CR536_RS28555 73784..74578 - 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
CR536_RS28560 74607..76037 - 1431 Protein_76 IS4-like element IS50R family transposase -
CR536_RS28565 76157..76345 + 189 WP_000957857.1 hypothetical protein -
CR536_RS28570 76355..77553 + 1199 Protein_78 IS91 family transposase -
CR536_RS29785 77719..77811 + 93 Protein_79 IS6 family transposase -
CR536_RS28580 78050..78331 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR536_RS28585 78321..78572 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
CR536_RS28595 79807..80646 + 840 WP_024129959.1 replication initiation protein -
CR536_RS28600 80667..81509 + 843 WP_000435064.1 replication initiation protein -
CR536_RS28605 81513..81959 + 447 WP_001074384.1 hypothetical protein -
CR536_RS28610 82027..82449 + 423 WP_179292036.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aac(3)-IId / ant(3'')-Ia / cmlA1 / aadA2 / tet(A) / sul3 / dfrA12 / oqxA / oqxB / aph(3')-IIa - 1..93781 93781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T86860 WP_000220561.1 NZ_CP024136:c78331-78050 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T86860 NZ_CP024136:c78331-78050 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT86860 WP_000121743.1 NZ_CP024136:c78572-78321 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT86860 NZ_CP024136:c78572-78321 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References