Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 54531..55024 Replicon plasmid p14EC017a
Accession NZ_CP024135
Organism Escherichia coli strain 14EC017

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag CR536_RS28070 Protein ID WP_001230709.1
Coordinates 54770..55024 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag CR536_RS28065 Protein ID WP_000151456.1
Coordinates 54531..54773 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR536_RS28020 49755..50462 + 708 WP_021503228.1 hypothetical protein -
CR536_RS28025 50482..50922 + 441 WP_023223352.1 hypothetical protein -
CR536_RS28030 50924..51250 + 327 WP_000206697.1 hypothetical protein -
CR536_RS28035 51510..51809 - 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
CR536_RS28040 52311..52493 + 183 WP_001428470.1 hypothetical protein -
CR536_RS28045 52581..52928 - 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
CR536_RS28050 52925..53173 - 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
CR536_RS28055 53667..53960 - 294 WP_000673428.1 hypothetical protein -
CR536_RS28065 54531..54773 - 243 WP_000151456.1 hypothetical protein Antitoxin
CR536_RS28070 54770..55024 - 255 WP_001230709.1 hypothetical protein Toxin
CR536_RS28075 55021..55563 - 543 WP_001083904.1 hypothetical protein -
CR536_RS28080 55667..56182 - 516 WP_001025393.1 J domain-containing protein -
CR536_RS28085 56586..56915 - 330 WP_000855119.1 hypothetical protein -
CR536_RS28095 57504..58169 + 666 WP_000532148.1 P-loop NTPase -
CR536_RS29950 58243..58527 + 285 WP_001162371.1 hypothetical protein -
CR536_RS28105 58545..59141 - 597 WP_000125165.1 hypothetical protein -
CR536_RS28110 59291..59452 - 162 WP_000775236.1 type I toxin-antitoxin system Hok family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..63978 63978


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Small toxic protein BsrE-like

(30-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T86859 WP_001230709.1 NZ_CP024135:c55024-54770 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T86859 NZ_CP024135:c55024-54770 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT86859 WP_000151456.1 NZ_CP024135:c54773-54531 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT86859 NZ_CP024135:c54773-54531 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References