Detailed information of TA system TA086858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 52581..53173
• Replicon: plasmid p14EC017a
• Accession: NZ_CP024135
• Organism: Escherichia coli strain 14EC017

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: CR536_RS28050
• Protein ID: WP_000513532.1
• Coordinates: 52925..53173 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: CR536_RS28045
• Protein ID: WP_000681609.1
• Coordinates: 52581..52928 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR536_RS28005	47873..48214	-	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-
CR536_RS28010	48582..48983	+	402	WP_021575837.1	hypothetical protein	-
CR536_RS28015	49001..49753	+	753	WP_000797966.1	conjugal transfer protein TraL	-
CR536_RS28020	49755..50462	+	708	WP_021503228.1	hypothetical protein	-
CR536_RS28025	50482..50922	+	441	WP_023223352.1	hypothetical protein	-
CR536_RS28030	50924..51250	+	327	WP_000206697.1	hypothetical protein	-
CR536_RS28035	51510..51809	-	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
CR536_RS28040	52311..52493	+	183	WP_001428470.1	hypothetical protein	-
CR536_RS28045	52581..52928	-	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CR536_RS28050	52925..53173	-	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
CR536_RS28055	53667..53960	-	294	WP_000673428.1	hypothetical protein	-
CR536_RS28065	54531..54773	-	243	WP_000151456.1	hypothetical protein	-
CR536_RS28070	54770..55024	-	255	WP_001230709.1	hypothetical protein	-
CR536_RS28075	55021..55563	-	543	WP_001083904.1	hypothetical protein	-
CR536_RS28080	55667..56182	-	516	WP_001025393.1	J domain-containing protein	-
CR536_RS28085	56586..56915	-	330	WP_000855119.1	hypothetical protein	-
CR536_RS28095	57504..58169	+	666	WP_000532148.1	P-loop NTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..63978	63978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T86858 WP_000513532.1 NZ_CP024135:c53173-52925 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T86858 NZ_CP024135:c53173-52925 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT86858 WP_000681609.1 NZ_CP024135:c52928-52581 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT86858 NZ_CP024135:c52928-52581 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49