Detailed information of TA system TA086857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 8612..9134
• Replicon: plasmid p14EC017a
• Accession: NZ_CP024135
• Organism: Escherichia coli strain 14EC017

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: CR536_RS27810
• Protein ID: WP_000638823.1
• Coordinates: 8853..9134 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: CR536_RS27805
• Protein ID: WP_000121741.1
• Coordinates: 8612..8863 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR536_RS29945	3681..3851	+	171	WP_000550720.1	hypothetical protein	-
CR536_RS27760	3862..4074	+	213	WP_039022940.1	hypothetical protein	-
CR536_RS27770	4345..4647	+	303	WP_001360345.1	hypothetical protein	-
CR536_RS27775	4644..4901	+	258	WP_000739144.1	hypothetical protein	-
CR536_RS27780	4905..5900	+	996	WP_001028541.1	type IV secretion system protein	-
CR536_RS27785	5906..6550	+	645	WP_001310442.1	conjugal transfer protein TrbJ	-
CR536_RS27790	6559..6783	+	225	WP_000713561.1	EexN family lipoprotein	-
CR536_RS27795	6997..7829	-	833	Protein_12	DNA primase	-
CR536_RS27800	7877..8512	+	636	WP_015059536.1	hypothetical protein	-
CR536_RS27805	8612..8863	+	252	WP_000121741.1	hypothetical protein	Antitoxin
CR536_RS27810	8853..9134	+	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR536_RS27815	9202..9501	+	300	WP_000835764.1	IncN plasmid KikA protein	-
CR536_RS27820	9504..10739	+	1236	WP_015059538.1	TcpQ domain-containing protein	-
CR536_RS27825	10745..11182	+	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
CR536_RS27830	11301..11699	+	399	WP_001153665.1	hypothetical protein	-
CR536_RS27835	11720..12304	+	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
CR536_RS27840	12406..12594	+	189	WP_050567133.1	hypothetical protein	-
CR536_RS27845	12665..12985	+	321	WP_000362080.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..63978	63978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T86857 WP_000638823.1 NZ_CP024135:8853-9134 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T86857 NZ_CP024135:8853-9134 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT86857 WP_000121741.1 NZ_CP024135:8612-8863 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT86857 NZ_CP024135:8612-8863 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1