Detailed information of TA system TA086855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4387476..4388052
• Replicon: chromosome
• Accession: NZ_CP024134
• Organism: Escherichia coli strain 14EC017

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: CR536_RS23560
• Protein ID: WP_001297643.1
• Coordinates: 4387765..4388052 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CR536_RS23555
• Protein ID: WP_101979790.1
• Coordinates: 4387476..4387778 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR536_RS23540	4384072..4386222	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
CR536_RS23545	4386272..4386475	+	204	WP_000467859.1	YbdD/YjiX family protein	-
CR536_RS23550	4386486..4387442	+	957	WP_001297640.1	GTPase	-
CR536_RS23555	4387476..4387778	-	303	WP_101979790.1	BrnA antitoxin family protein	Antitoxin
CR536_RS23560	4387765..4388052	-	288	WP_001297643.1	BrnT family toxin	Toxin
CR536_RS23565	4388251..4388948	-	698	Protein_4287	IS1 family transposase	-
CR536_RS23570	4389005..4389100	+	96	Protein_4288	dienelactone hydrolase family protein	-
CR536_RS23575	4389246..4390478	+	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
CR536_RS23580	4390519..4391799	+	1281	WP_001037380.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T86855 WP_001297643.1 NZ_CP024134:c4388052-4387765 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T86855 NZ_CP024134:c4388052-4387765 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11536.06 Da
• Isoelectric Point: 9.7969

>AT86855 WP_101979790.1 NZ_CP024134:c4387778-4387476 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLKAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT86855 NZ_CP024134:c4387778-4387476 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAAGGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9