Detailed information of TA system TA086850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2385295..2385821
• Replicon: chromosome
• Accession: NZ_CP024134
• Organism: Escherichia coli strain 14EC017

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CR536_RS12735
• Protein ID: WP_000323025.1
• Coordinates: 2385534..2385821 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CR536_RS12730
• Protein ID: WP_000534858.1
• Coordinates: 2385295..2385534 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR536_RS12695	2381309..2382006	+	698	Protein_2304	IS1 family transposase	-
CR536_RS12710	2382680..2383993	+	1314	WP_000589009.1	ISNCY family transposase	-
CR536_RS12720	2384430..2384762	-	333	WP_001301033.1	protein FlxA	-
CR536_RS12725	2384965..2385270	-	306	WP_001326990.1	hypothetical protein	-
CR536_RS12730	2385295..2385534	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CR536_RS12735	2385534..2385821	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CR536_RS12740	2385893..2386048	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CR536_RS12745	2386265..2386516	+	252	WP_000980994.1	hypothetical protein	-
CR536_RS12750	2386583..2386861	+	279	WP_012304870.1	hypothetical protein	-
CR536_RS12755	2386863..2387912	+	1050	WP_096174476.1	DUF968 domain-containing protein	-
CR536_RS12760	2387926..2388678	+	753	WP_001047136.1	antitermination protein	-
CR536_RS29505	2388956..2389045	-	90	WP_120795389.1	hypothetical protein	-
CR536_RS12765	2389100..2389312	-	213	WP_000087757.1	cold shock-like protein CspF	-
CR536_RS12770	2389613..2389828	+	216	WP_000066484.1	cold shock-like protein CspB	-
CR536_RS30040	2390192..2390362	-	171	WP_001405948.1	putative zinc-binding protein YnfU	-
CR536_RS12780	2390582..2390797	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2378339..2430330	51991
	-	flank	IS/Tn	-	-	2381503..2382006	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T86850 WP_000323025.1 NZ_CP024134:2385534-2385821 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T86850 NZ_CP024134:2385534-2385821 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT86850 WP_000534858.1 NZ_CP024134:2385295-2385534 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT86850 NZ_CP024134:2385295-2385534 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8