Detailed information of TA system TA086841

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1950162..1950994
• Replicon: chromosome
• Accession: NZ_CP024134
• Organism: Escherichia coli strain 14EC017

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: CR536_RS10325
• Protein ID: WP_000854765.1
• Coordinates: 1950162..1950536 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: CR536_RS10330
• Protein ID: WP_001315620.1
• Coordinates: 1950626..1950994 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR536_RS29840	1946052..1946423	-	372	WP_001445978.1	IS110 family transposase	-
CR536_RS10310	1947259..1948572	+	1314	Protein_1877	IS21-like element ISEc12 family transposase	-
CR536_RS10320	1949959..1950165	-	207	WP_000976829.1	hypothetical protein	-
CR536_RS10325	1950162..1950536	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
CR536_RS10330	1950626..1950994	-	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CR536_RS10335	1951157..1951378	-	222	WP_000692345.1	DUF987 domain-containing protein	-
CR536_RS10340	1951441..1951917	-	477	WP_001186774.1	RadC family protein	-
CR536_RS10345	1951933..1952406	-	474	WP_000855059.1	antirestriction protein	-
CR536_RS10350	1952443..1954056	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
CR536_RS10355	1954087..1954437	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
CR536_RS10360	1954434..1954808	-	375	Protein_1887	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1934533..1960649	26116
	-	inside	IScluster/Tn	-	-	1942600..1956072	13472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T86841 WP_000854765.1 NZ_CP024134:c1950536-1950162 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T86841 NZ_CP024134:c1950536-1950162 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT86841 WP_001315620.1 NZ_CP024134:c1950994-1950626 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT86841 NZ_CP024134:c1950994-1950626 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: V0ULY5