Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1155162..1155997 Replicon chromosome
Accession NZ_CP024134
Organism Escherichia coli strain 14EC017

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J5ZPW7
Locus tag CR536_RS06080 Protein ID WP_000854722.1
Coordinates 1155162..1155539 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0K4A940
Locus tag CR536_RS06085 Protein ID WP_001285576.1
Coordinates 1155629..1155997 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR536_RS06050 1150544..1152795 - 2252 Protein_1101 alpha-amylase family protein -
CR536_RS06065 1153530..1154375 - 846 WP_001280427.1 DUF4942 domain-containing protein -
CR536_RS06070 1154460..1154657 - 198 WP_000445281.1 DUF957 domain-containing protein -
CR536_RS06075 1154677..1155165 - 489 WP_000761669.1 hypothetical protein -
CR536_RS06080 1155162..1155539 - 378 WP_000854722.1 TA system toxin CbtA family protein Toxin
CR536_RS06085 1155629..1155997 - 369 WP_001285576.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CR536_RS06090 1156077..1156298 - 222 WP_000692176.1 DUF987 domain-containing protein -
CR536_RS06095 1156385..1156861 - 477 WP_001360063.1 RadC family protein -
CR536_RS06105 1156877..1157362 - 486 WP_000206656.1 antirestriction protein -
CR536_RS06110 1157454..1158272 - 819 WP_024220054.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1152631..1191659 39028


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14103.15 Da        Isoelectric Point: 8.2904

>T86839 WP_000854722.1 NZ_CP024134:c1155539-1155162 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T86839 NZ_CP024134:c1155539-1155162 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13599.36 Da        Isoelectric Point: 6.3159

>AT86839 WP_001285576.1 NZ_CP024134:c1155997-1155629 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT86839 NZ_CP024134:c1155997-1155629 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGTGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J5ZPW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0K4A940

References