Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 19050..19585 Replicon plasmid p14EC007b
Accession NZ_CP024133
Organism Escherichia coli strain 14EC007

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag CR535_RS27530 Protein ID WP_000222766.1
Coordinates 19298..19585 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag CR535_RS27525 Protein ID WP_001132900.1
Coordinates 19050..19301 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR535_RS27485 14712..15227 + 516 WP_000998852.1 type II secretion system protein M -
CR535_RS27490 15279..16112 + 834 WP_000088802.1 A24 family peptidase -
CR535_RS27495 16206..16607 + 402 WP_001222320.1 GspS family T2SS pilot lipoprotein variant EptO -
CR535_RS27500 16682..16897 + 216 Protein_16 IS3 family transposase -
CR535_RS29035 17196..17489 + 294 Protein_17 hypothetical protein -
CR535_RS27515 17718..17966 + 249 WP_000083841.1 replication regulatory protein RepA -
CR535_RS29040 17989..18096 + 108 Protein_19 incFII family plasmid replication initiator RepA -
CR535_RS27525 19050..19301 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CR535_RS27530 19298..19585 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / sul3 / blaTEM-1B / qnrS1 / dfrA12 / aadA2 / tet(A) stcE / exeE / exeG 1..190293 190293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T86829 WP_000222766.1 NZ_CP024133:19298-19585 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T86829 NZ_CP024133:19298-19585 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT86829 WP_001132900.1 NZ_CP024133:19050-19301 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT86829 NZ_CP024133:19050-19301 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References