Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 1196..1661 Replicon plasmid p14EC007a
Accession NZ_CP024132
Organism Escherichia coli strain 14EC007

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag CR535_RS27170 Protein ID WP_001230707.1
Coordinates 1196..1447 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag CR535_RS27175 Protein ID WP_000801440.1
Coordinates 1434..1661 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR535_RS27160 37..552 + 516 WP_001025395.1 J domain-containing protein -
CR535_RS27165 657..1199 + 543 WP_001083906.1 hypothetical protein -
CR535_RS27170 1196..1447 + 252 WP_001230707.1 hypothetical protein Toxin
CR535_RS27175 1434..1661 + 228 WP_000801440.1 hypothetical protein Antitoxin
CR535_RS27185 2115..2414 + 300 WP_000650309.1 hypothetical protein -
CR535_RS27190 2505..2855 + 351 WP_000854259.1 hypothetical protein -
CR535_RS27195 3160..3408 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
CR535_RS27200 3405..3752 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
CR535_RS27210 4113..4616 + 504 WP_000781813.1 hypothetical protein -
CR535_RS27215 4620..5849 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
CR535_RS27220 5926..6219 + 294 WP_060598542.1 LuxR family transcriptional regulator -
CR535_RS27225 6216..6431 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..35098 35098


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T86827 WP_001230707.1 NZ_CP024132:1196-1447 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T86827 NZ_CP024132:1196-1447 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT86827 WP_000801440.1 NZ_CP024132:1434-1661 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT86827 NZ_CP024132:1434-1661 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References