Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3760672..3761290 Replicon chromosome
Accession NZ_CP024131
Organism Escherichia coli strain 14EC007

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CR535_RS20165 Protein ID WP_001291435.1
Coordinates 3761072..3761290 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CR535_RS20160 Protein ID WP_000344800.1
Coordinates 3760672..3761046 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR535_RS20145 3756201..3759350 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CR535_RS20155 3759544..3760241 + 698 Protein_3669 IS1 family transposase -
CR535_RS20160 3760672..3761046 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CR535_RS20165 3761072..3761290 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CR535_RS20170 3761462..3762013 + 552 WP_000102564.1 maltose O-acetyltransferase -
CR535_RS20175 3762129..3762599 + 471 WP_000136192.1 YlaC family protein -
CR535_RS20180 3762763..3764313 + 1551 WP_101973075.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CR535_RS20185 3764355..3764708 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CR535_RS20195 3765087..3765398 + 312 WP_000409911.1 MGMT family protein -
CR535_RS20200 3765429..3766001 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T86823 WP_001291435.1 NZ_CP024131:3761072-3761290 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T86823 NZ_CP024131:3761072-3761290 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT86823 WP_000344800.1 NZ_CP024131:3760672-3761046 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT86823 NZ_CP024131:3760672-3761046 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References