Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2400669..2401195 Replicon chromosome
Accession NZ_CP024131
Organism Escherichia coli strain 14EC007

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CR535_RS12815 Protein ID WP_000323025.1
Coordinates 2400908..2401195 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CR535_RS12810 Protein ID WP_000534858.1
Coordinates 2400669..2400908 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR535_RS12780 2397354..2397518 - 165 WP_101972892.1 protein FlxA -
CR535_RS12785 2397562..2397942 + 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
CR535_RS12790 2397939..2398286 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
CR535_RS12795 2398336..2399874 + 1539 WP_053888474.1 IS66-like element ISEc8 family transposase -
CR535_RS12800 2399912..2400136 - 225 Protein_2320 FlxA-like family protein -
CR535_RS12805 2400339..2400644 - 306 WP_001326990.1 hypothetical protein -
CR535_RS12810 2400669..2400908 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CR535_RS12815 2400908..2401195 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CR535_RS12820 2401267..2401422 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CR535_RS12825 2401639..2401890 + 252 WP_099949844.1 hypothetical protein -
CR535_RS12830 2401957..2402235 + 279 WP_012304870.1 hypothetical protein -
CR535_RS12835 2402237..2403286 + 1050 WP_099949845.1 DUF968 domain-containing protein -
CR535_RS12840 2403300..2404052 + 753 WP_101972893.1 antitermination protein -
CR535_RS28705 2404330..2404419 - 90 WP_120795389.1 hypothetical protein -
CR535_RS12845 2404474..2404686 - 213 WP_000087756.1 cold shock-like protein CspF -
CR535_RS12850 2404987..2405202 + 216 WP_000066484.1 cold shock-like protein CspB -
CR535_RS29130 2405566..2405736 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
CR535_RS12855 2405956..2406171 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2379753..2451584 71831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T86820 WP_000323025.1 NZ_CP024131:2400908-2401195 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T86820 NZ_CP024131:2400908-2401195 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT86820 WP_000534858.1 NZ_CP024131:2400669-2400908 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT86820 NZ_CP024131:2400669-2400908 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References