Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59938..60581 Replicon plasmid p14EC001c
Accession NZ_CP024130
Organism Escherichia coli strain 14EC001

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag CR534_RS28655 Protein ID WP_001044768.1
Coordinates 60165..60581 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag CR534_RS28650 Protein ID WP_001261287.1
Coordinates 59938..60168 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR534_RS28610 55223..56917 - 1695 WP_000105636.1 mercury(II) reductase -
CR534_RS28615 56969..57391 - 423 WP_001340589.1 organomercurial transporter MerC -
CR534_RS28620 57427..57702 - 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
CR534_RS28625 57716..58066 - 351 WP_001294663.1 mercuric transport protein MerT -
CR534_RS28630 58138..58572 + 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
CR534_RS28635 58619..59323 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
CR534_RS28640 59370..59642 - 273 WP_061602529.1 hypothetical protein -
CR534_RS28650 59938..60168 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CR534_RS28655 60165..60581 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
CR534_RS28660 60743..62881 - 2139 WP_101981714.1 AAA family ATPase -
CR534_RS28665 63235..63492 + 258 WP_000343085.1 hypothetical protein -
CR534_RS28670 63492..64082 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid oqxB / oqxA / sul3 / mef(B) / aph(3')-Ia - 1..88460 88460
- flank IS/Tn - - 58619..59323 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T86807 WP_001044768.1 NZ_CP024130:60165-60581 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T86807 NZ_CP024130:60165-60581 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT86807 WP_001261287.1 NZ_CP024130:59938-60168 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT86807 NZ_CP024130:59938-60168 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References