Detailed information of TA system TA086786

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1869568..1870400
• Replicon: chromosome
• Accession: NZ_CP024127
• Organism: Escherichia coli strain 14EC001

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1L4HFA4
• Locus tag: CR534_RS09795
• Protein ID: WP_032163901.1
• Coordinates: 1869568..1869942 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CR534_RS09800
• Protein ID: WP_040089920.1
• Coordinates: 1870032..1870400 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR534_RS09760	1864979..1866145	+	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
CR534_RS09765	1866264..1866737	+	474	Protein_1784	DNA gyrase inhibitor SbmC	-
CR534_RS09770	1866935..1867993	+	1059	WP_001200910.1	FUSC family protein	-
CR534_RS09775	1868165..1868494	+	330	WP_000450409.1	DUF496 family protein	-
CR534_RS09780	1868595..1868777	-	183	WP_001016348.1	hypothetical protein	-
CR534_RS09785	1868849..1868977	+	129	Protein_1788	transposase domain-containing protein	-
CR534_RS29260	1869266..1869379	-	114	WP_001161660.1	DUF957 domain-containing protein	-
CR534_RS09790	1869392..1869571	-	180	WP_053902470.1	hypothetical protein	-
CR534_RS09795	1869568..1869942	-	375	WP_032163901.1	TA system toxin CbtA family protein	Toxin
CR534_RS09800	1870032..1870400	-	369	WP_040089920.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
CR534_RS09805	1870474..1870695	-	222	WP_000692323.1	DUF987 domain-containing protein	-
CR534_RS09810	1870764..1871240	-	477	WP_001186725.1	RadC family protein	-
CR534_RS09815	1871256..1871741	-	486	WP_097514179.1	antirestriction protein	-
CR534_RS09820	1871796..1872614	-	819	WP_001234620.1	DUF945 domain-containing protein	-
CR534_RS09830	1872714..1872947	-	234	WP_001119729.1	DUF905 family protein	-
CR534_RS09835	1873026..1873481	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13919.89 Da
• Isoelectric Point: 7.7760

>T86786 WP_032163901.1 NZ_CP024127:c1869942-1869568 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T86786 NZ_CP024127:c1869942-1869568 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13529.45 Da
• Isoelectric Point: 6.6290

>AT86786 WP_040089920.1 NZ_CP024127:c1870400-1870032 [Escherichia coli]
VSDTLPGTILPDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT86786 NZ_CP024127:c1870400-1870032 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAATACTTCCCGACGACAATCACGACCGCCTCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L4HFA4