Detailed information of TA system TA086765

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 6215..6945
• Replicon: plasmid pCh1_67
• Accession: NZ_CP024122
• Organism: Bacillus cytotoxicus strain CH_1

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: CG474_RS22095
• Protein ID: WP_094374045.1
• Coordinates: 6215..6787 (-)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	6849..6945 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG474_RS22075	1665..2019	-	355	Protein_4	CPBP family intramembrane metalloprotease	-
CG474_RS23075	2184..3431	-	1248	Protein_5	topoisomerase C-terminal repeat-containing protein	-
CG474_RS23080	3491..4277	-	787	Protein_6	hypothetical protein	-
CG474_RS22085	4424..5314	-	891	WP_094398400.1	hypothetical protein	-
CG474_RS22090	5686..6066	-	381	WP_094374043.1	hypothetical protein	-
CG474_RS22095	6215..6787	-	573	WP_094374045.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
-	6849..6945	-	97	NuclAT_0	-	Antitoxin
-	6849..6945	-	97	NuclAT_0	-	Antitoxin
-	6849..6945	-	97	NuclAT_0	-	Antitoxin
-	6849..6945	-	97	NuclAT_0	-	Antitoxin
CG474_RS22100	7049..8071	-	1023	WP_094374047.1	DUF3991 and TOPRIM domain-containing protein	-
CG474_RS22105	8199..8750	-	552	WP_094374049.1	metal-dependent hydrolase	-
CG474_RS22110	8775..9755	-	981	WP_094374051.1	hypothetical protein	-
CG474_RS22115	9862..11631	-	1770	WP_162533114.1	hypothetical protein	-
CG474_RS22120	11628..11837	-	210	WP_094374055.1	DUF5511 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67308	67308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22516.91 Da
• Isoelectric Point: 9.5095

>T86765 WP_094374045.1 NZ_CP024122:c6787-6215 [Bacillus cytotoxicus]
MANKDNPKFYTISTKYIDYLRETDNKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDKDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKMETDPKYGNLMLKQYLFLKENMDRLSHKVEKVYKDVTVQGKPSKKQKFLKGICCD
FSTLEEKCQEYKEKNQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T86765 NZ_CP024122:c6787-6215 [Bacillus cytotoxicus]
TTGGCTAATAAAGATAATCCTAAATTCTATACGATTTCAACTAAATATATAGACTATCTAAGGGAAACAGATAATAAAGT
GCCCTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGCGTATTGGAAAAAATCAATGGTCATGATTATTTTGTTC
CTTTAACATCACGTAATGATAAAAACTTTAATAGCCAGGTCAGTGTGAAATTATTCGATAAAGATAACCCTGAAAAGCGA
ATTGGAGTTTTATTAGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAATGGA
GACAGATCCTAAGTATGGTAATTTGATGTTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGACTTTCTCATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCATCAAAAAAACAGAAATTTCTAAAGGGAATTTGCTGTGAC
TTCTCAACATTAGAAGAAAAGTGCCAGGAATATAAAGAGAAGAATCAAGCTAGAAGAATAGCATATATGCGTCAAATGGG
GCGTGAAAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT86765 NZ_CP024122:c6945-6849 [Bacillus cytotoxicus]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin