Detailed information of TA system TA086759

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 10368..11098
• Replicon: plasmid pCh2_83
• Accession: NZ_CP024118
• Organism: Bacillus cytotoxicus strain CH_2

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: CG476_RS22680
• Protein ID: WP_094374045.1
• Coordinates: 10526..11098 (+)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	10368..10464 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG476_RS22655	5470..5679	+	210	WP_094374055.1	DUF5511 family protein	-
CG476_RS23640	5676..7450	+	1775	Protein_9	hypothetical protein	-
CG476_RS22665	7557..8537	+	981	WP_094374051.1	hypothetical protein	-
CG476_RS22670	8562..9113	+	552	WP_094374049.1	metal-dependent hydrolase	-
CG476_RS22675	9260..10264	+	1005	WP_094398204.1	DUF3991 and TOPRIM domain-containing protein	-
-	10368..10464	+	97	NuclAT_0	-	Antitoxin
-	10368..10464	+	97	NuclAT_0	-	Antitoxin
-	10368..10464	+	97	NuclAT_0	-	Antitoxin
-	10368..10464	+	97	NuclAT_0	-	Antitoxin
CG476_RS22680	10526..11098	+	573	WP_094374045.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
CG476_RS22685	11247..11627	+	381	WP_094374043.1	hypothetical protein	-
CG476_RS22690	11979..12818	+	840	WP_063262609.1	heteromeric transposase endonuclease subunit TnsA	-
CG476_RS22695	12815..14971	+	2157	WP_063262610.1	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..83527	83527

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22516.91 Da
• Isoelectric Point: 9.5095

>T86759 WP_094374045.1 NZ_CP024118:10526-11098 [Bacillus cytotoxicus]
MANKDNPKFYTISTKYIDYLRETDNKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDKDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKMETDPKYGNLMLKQYLFLKENMDRLSHKVEKVYKDVTVQGKPSKKQKFLKGICCD
FSTLEEKCQEYKEKNQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T86759 NZ_CP024118:10526-11098 [Bacillus cytotoxicus]
TTGGCTAATAAAGATAATCCTAAATTCTATACGATTTCAACTAAATATATAGACTATCTAAGGGAAACAGATAATAAAGT
GCCCTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGCGTATTGGAAAAAATCAATGGTCATGATTATTTTGTTC
CTTTAACATCACGTAATGATAAAAACTTTAATAGCCAGGTCAGTGTGAAATTATTCGATAAAGATAACCCTGAAAAGCGA
ATTGGAGTTTTATTAGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAATGGA
GACAGATCCTAAGTATGGTAATTTGATGTTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGACTTTCTCATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCATCAAAAAAACAGAAATTTCTAAAGGGAATTTGCTGTGAC
TTCTCAACATTAGAAGAAAAGTGCCAGGAATATAAAGAGAAGAATCAAGCTAGAAGAATAGCATATATGCGTCAAATGGG
GCGTGAAAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT86759 NZ_CP024118:10368-10464 [Bacillus cytotoxicus]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin