Detailed information of TA system TA086758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 1999311..2000303
• Replicon: chromosome
• Accession: NZ_CP024116
• Organism: Bacillus cytotoxicus strain CH_2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A7GPN6
• Locus tag: CG476_RS10240
• Protein ID: WP_012094283.1
• Coordinates: 1999311..2000051 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A7GPN7
• Locus tag: CG476_RS10245
• Protein ID: WP_012094285.1
• Coordinates: 2000175..2000303 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG476_RS23395	1994451..1994716	+	266	Protein_1868	hypothetical protein	-
CG476_RS10210	1994786..1995688	+	903	WP_012094278.1	ATPase	-
CG476_RS10215	1995735..1996040	-	306	Protein_1870	hypothetical protein	-
CG476_RS10220	1996235..1997539	+	1305	WP_087094924.1	DEAD/DEAH box helicase	-
CG476_RS10225	1997759..1998241	+	483	WP_012094281.1	MarR family winged helix-turn-helix transcriptional regulator	-
CG476_RS10230	1998379..1999116	+	738	WP_012094282.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CG476_RS10240	1999311..2000051	+	741	WP_012094283.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CG476_RS10245	2000175..2000303	+	129	WP_012094285.1	hypothetical protein	Antitoxin
CG476_RS10250	2000380..2000556	+	177	WP_087094925.1	stage III sporulation protein AA	-
CG476_RS10255	2000715..2001260	-	546	WP_087094926.1	GNAT family N-acetyltransferase	-
CG476_RS10260	2001301..2002182	-	882	WP_087094927.1	aminoglycoside 6-adenylyltransferase	-
CG476_RS10265	2002388..2003857	+	1470	WP_087094928.1	aminoacyl-histidine dipeptidase	-
CG476_RS10270	2004456..2004707	+	252	WP_012094290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28628.30 Da
• Isoelectric Point: 6.7302

>T86758 WP_012094283.1 NZ_CP024116:1999311-2000051 [Bacillus cytotoxicus]
MTVTSVRIGLFFLVIFFLFLIFFYWRNEERYEERKQLIRKTWYGLFITSVTMYFMMKGIDLTLWKHILMVVAMVVFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTLIQNVNPAAFGTMEWETDAEYARSLNAFLDTYAEKIGAK
IVVFVTEEELNTNFRGVRSQFSITVSPESIEQLNEQKAVQIENVGIIPAKIIKDVYIIMDGQKNNLQDRDFENVYNLTIH
HSYFCK

Nucleotide

• Length: 741 bp

>T86758 NZ_CP024116:1999311-2000051 [Bacillus cytotoxicus]
TTGACTGTCACTAGCGTTCGAATTGGCTTGTTTTTTCTTGTAATCTTTTTTTTATTTCTTATTTTCTTTTATTGGAGAAA
TGAAGAACGATACGAGGAAAGAAAACAACTTATTCGAAAGACGTGGTATGGTTTATTTATTACATCAGTCACAATGTATT
TCATGATGAAAGGAATTGATTTAACCCTCTGGAAACATATTTTAATGGTTGTTGCGATGGTTGTATTTGTTGATATTGCA
TTTATTTTAACGCCAAATATTTCGGAAATCTGGGGCGCAAAGTTTAGTGATATTGGTAAGACAGTTCAATCGATTAAACG
ATCGCTTATTGCTTCAAAAGCAAGAGGAGAAATATATACAACACTTATTCAAAATGTAAATCCAGCTGCATTTGGTACGA
TGGAATGGGAAACAGATGCTGAGTATGCACGCAGTTTAAATGCTTTTCTTGATACGTATGCTGAAAAAATAGGTGCGAAA
ATTGTAGTATTTGTCACAGAAGAGGAATTAAATACAAACTTCCGAGGTGTTCGATCTCAATTTAGTATTACAGTATCACC
CGAATCTATTGAACAACTGAATGAGCAAAAAGCAGTTCAAATTGAAAATGTTGGAATTATACCAGCGAAAATAATAAAAG
ACGTTTATATTATTATGGATGGACAAAAAAATAATTTGCAAGATCGTGACTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTACTTTTGTAAGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4984.43 Da
• Isoelectric Point: 3.9944

>AT86758 WP_012094285.1 NZ_CP024116:2000175-2000303 [Bacillus cytotoxicus]
VLKEYSNDFFKDEAEDTTDFSLIKGDTMQQVEELEEELKRRG

Nucleotide

• Length: 129 bp

>AT86758 NZ_CP024116:2000175-2000303 [Bacillus cytotoxicus]
GTGTTAAAAGAATATAGCAATGATTTCTTTAAAGACGAAGCTGAGGATACAACAGATTTTTCTTTAATAAAAGGAGATAC
AATGCAGCAAGTGGAAGAATTAGAAGAAGAATTGAAAAGAAGAGGATAA

Structures

Toxin

• AlphaFold DB: A7GPN6

Antitoxin

• AlphaFold DB: A7GPN7