Detailed information of TA system TA086747

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 20357..21087
• Replicon: plasmid pCh4_83
• Accession: NZ_CP024112
• Organism: Bacillus cytotoxicus strain CH_4

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: CG478_RS22225
• Protein ID: WP_094374045.1
• Coordinates: 20357..20929 (-)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	20991..21087 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG478_RS22210	16484..18640	-	2157	WP_063262610.1	DDE-type integrase/transposase/recombinase	-
CG478_RS22215	18637..19476	-	840	WP_063262609.1	heteromeric transposase endonuclease subunit TnsA	-
CG478_RS22220	19828..20208	-	381	WP_094374043.1	hypothetical protein	-
CG478_RS22225	20357..20929	-	573	WP_094374045.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
-	20991..21087	-	97	NuclAT_0	-	Antitoxin
-	20991..21087	-	97	NuclAT_0	-	Antitoxin
-	20991..21087	-	97	NuclAT_0	-	Antitoxin
-	20991..21087	-	97	NuclAT_0	-	Antitoxin
CG478_RS22230	21191..22213	-	1023	WP_094374047.1	DUF3991 and TOPRIM domain-containing protein	-
CG478_RS22235	22341..22892	-	552	WP_094374049.1	metal-dependent hydrolase	-
CG478_RS22240	22917..23897	-	981	WP_094374051.1	hypothetical protein	-
CG478_RS22245	24004..25773	-	1770	WP_162533114.1	hypothetical protein	-
CG478_RS22250	25770..25979	-	210	WP_094374055.1	DUF5511 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..83510	83510

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22516.91 Da
• Isoelectric Point: 9.5095

>T86747 WP_094374045.1 NZ_CP024112:c20929-20357 [Bacillus cytotoxicus]
MANKDNPKFYTISTKYIDYLRETDNKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDKDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKMETDPKYGNLMLKQYLFLKENMDRLSHKVEKVYKDVTVQGKPSKKQKFLKGICCD
FSTLEEKCQEYKEKNQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T86747 NZ_CP024112:c20929-20357 [Bacillus cytotoxicus]
TTGGCTAATAAAGATAATCCTAAATTCTATACGATTTCAACTAAATATATAGACTATCTAAGGGAAACAGATAATAAAGT
GCCCTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGCGTATTGGAAAAAATCAATGGTCATGATTATTTTGTTC
CTTTAACATCACGTAATGATAAAAACTTTAATAGCCAGGTCAGTGTGAAATTATTCGATAAAGATAACCCTGAAAAGCGA
ATTGGAGTTTTATTAGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAATGGA
GACAGATCCTAAGTATGGTAATTTGATGTTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGACTTTCTCATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCATCAAAAAAACAGAAATTTCTAAAGGGAATTTGCTGTGAC
TTCTCAACATTAGAAGAAAAGTGCCAGGAATATAAAGAGAAGAATCAAGCTAGAAGAATAGCATATATGCGTCAAATGGG
GCGTGAAAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT86747 NZ_CP024112:c21087-20991 [Bacillus cytotoxicus]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin