Detailed information of TA system TA086746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 931955..932947
• Replicon: chromosome
• Accession: NZ_CP024111
• Organism: Bacillus cytotoxicus strain CH_4

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A7GPN6
• Locus tag: CG478_RS05025
• Protein ID: WP_012094283.1
• Coordinates: 932207..932947 (-)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A7GPN7
• Locus tag: CG478_RS05020
• Protein ID: WP_012094285.1
• Coordinates: 931955..932083 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG478_RS04995	927551..927802	-	252	WP_012094290.1	hypothetical protein	-
CG478_RS05000	928401..929870	-	1470	WP_087094928.1	aminoacyl-histidine dipeptidase	-
CG478_RS05005	930076..930957	+	882	WP_087094927.1	aminoglycoside 6-adenylyltransferase	-
CG478_RS05010	930998..931543	+	546	WP_087094926.1	GNAT family N-acetyltransferase	-
CG478_RS05015	931702..931878	-	177	WP_087094925.1	stage III sporulation protein AA	-
CG478_RS05020	931955..932083	-	129	WP_012094285.1	hypothetical protein	Antitoxin
CG478_RS05025	932207..932947	-	741	WP_012094283.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CG478_RS05035	933142..933879	-	738	WP_012094282.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CG478_RS05040	934017..934499	-	483	WP_012094281.1	MarR family winged helix-turn-helix transcriptional regulator	-
CG478_RS05045	934719..936023	-	1305	WP_087094924.1	DEAD/DEAH box helicase	-
CG478_RS05050	936218..936523	+	306	Protein_922	hypothetical protein	-
CG478_RS05055	936570..937472	-	903	WP_012094278.1	ATPase	-
CG478_RS22870	937542..937807	-	266	Protein_924	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28628.30 Da
• Isoelectric Point: 6.7302

>T86746 WP_012094283.1 NZ_CP024111:c932947-932207 [Bacillus cytotoxicus]
MTVTSVRIGLFFLVIFFLFLIFFYWRNEERYEERKQLIRKTWYGLFITSVTMYFMMKGIDLTLWKHILMVVAMVVFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTLIQNVNPAAFGTMEWETDAEYARSLNAFLDTYAEKIGAK
IVVFVTEEELNTNFRGVRSQFSITVSPESIEQLNEQKAVQIENVGIIPAKIIKDVYIIMDGQKNNLQDRDFENVYNLTIH
HSYFCK

Nucleotide

• Length: 741 bp

>T86746 NZ_CP024111:c932947-932207 [Bacillus cytotoxicus]
TTGACTGTCACTAGCGTTCGAATTGGCTTGTTTTTTCTTGTAATCTTTTTTTTATTTCTTATTTTCTTTTATTGGAGAAA
TGAAGAACGATACGAGGAAAGAAAACAACTTATTCGAAAGACGTGGTATGGTTTATTTATTACATCAGTCACAATGTATT
TCATGATGAAAGGAATTGATTTAACCCTCTGGAAACATATTTTAATGGTTGTTGCGATGGTTGTATTTGTTGATATTGCA
TTTATTTTAACGCCAAATATTTCGGAAATCTGGGGCGCAAAGTTTAGTGATATTGGTAAGACAGTTCAATCGATTAAACG
ATCGCTTATTGCTTCAAAAGCAAGAGGAGAAATATATACAACACTTATTCAAAATGTAAATCCAGCTGCATTTGGTACGA
TGGAATGGGAAACAGATGCTGAGTATGCACGCAGTTTAAATGCTTTTCTTGATACGTATGCTGAAAAAATAGGTGCGAAA
ATTGTAGTATTTGTCACAGAAGAGGAATTAAATACAAACTTCCGAGGTGTTCGATCTCAATTTAGTATTACAGTATCACC
CGAATCTATTGAACAACTGAATGAGCAAAAAGCAGTTCAAATTGAAAATGTTGGAATTATACCAGCGAAAATAATAAAAG
ACGTTTATATTATTATGGATGGACAAAAAAATAATTTGCAAGATCGTGACTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTACTTTTGTAAGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4984.43 Da
• Isoelectric Point: 3.9944

>AT86746 WP_012094285.1 NZ_CP024111:c932083-931955 [Bacillus cytotoxicus]
VLKEYSNDFFKDEAEDTTDFSLIKGDTMQQVEELEEELKRRG

Nucleotide

• Length: 129 bp

>AT86746 NZ_CP024111:c932083-931955 [Bacillus cytotoxicus]
GTGTTAAAAGAATATAGCAATGATTTCTTTAAAGACGAAGCTGAGGATACAACAGATTTTTCTTTAATAAAAGGAGATAC
AATGCAGCAAGTGGAAGAATTAGAAGAAGAATTGAAAAGAAGAGGATAA

Structures

Toxin

• AlphaFold DB: A7GPN6

Antitoxin

• AlphaFold DB: A7GPN7