Detailed information of TA system TA086729

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 28902..29632
• Replicon: plasmid pCh23_67
• Accession: NZ_CP024106
• Organism: Bacillus cytotoxicus strain CH_23

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: CG481_RS22485
• Protein ID: WP_094374045.1
• Coordinates: 29060..29632 (+)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	28902..28998 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG481_RS22460	24010..24219	+	210	WP_094374055.1	DUF5511 family protein	-
CG481_RS22465	24216..25985	+	1770	WP_162533114.1	hypothetical protein	-
CG481_RS22470	26092..27072	+	981	WP_094374051.1	hypothetical protein	-
CG481_RS22475	27097..27648	+	552	WP_094374049.1	metal-dependent hydrolase	-
CG481_RS22480	27776..28798	+	1023	WP_094374047.1	DUF3991 and TOPRIM domain-containing protein	-
-	28902..28998	+	97	NuclAT_0	-	Antitoxin
-	28902..28998	+	97	NuclAT_0	-	Antitoxin
-	28902..28998	+	97	NuclAT_0	-	Antitoxin
-	28902..28998	+	97	NuclAT_0	-	Antitoxin
CG481_RS22485	29060..29632	+	573	WP_094374045.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
CG481_RS22490	29781..30161	+	381	WP_094374043.1	hypothetical protein	-
CG481_RS22495	30533..31468	+	936	WP_094396514.1	hypothetical protein	-
CG481_RS22500	31561..33714	+	2154	WP_094396515.1	type IA DNA topoisomerase	-
CG481_RS22505	33746..34195	+	450	WP_162533120.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67307	67307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22516.91 Da
• Isoelectric Point: 9.5095

>T86729 WP_094374045.1 NZ_CP024106:29060-29632 [Bacillus cytotoxicus]
MANKDNPKFYTISTKYIDYLRETDNKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDKDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKMETDPKYGNLMLKQYLFLKENMDRLSHKVEKVYKDVTVQGKPSKKQKFLKGICCD
FSTLEEKCQEYKEKNQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T86729 NZ_CP024106:29060-29632 [Bacillus cytotoxicus]
TTGGCTAATAAAGATAATCCTAAATTCTATACGATTTCAACTAAATATATAGACTATCTAAGGGAAACAGATAATAAAGT
GCCCTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGCGTATTGGAAAAAATCAATGGTCATGATTATTTTGTTC
CTTTAACATCACGTAATGATAAAAACTTTAATAGCCAGGTCAGTGTGAAATTATTCGATAAAGATAACCCTGAAAAGCGA
ATTGGAGTTTTATTAGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAATGGA
GACAGATCCTAAGTATGGTAATTTGATGTTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGACTTTCTCATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCATCAAAAAAACAGAAATTTCTAAAGGGAATTTGCTGTGAC
TTCTCAACATTAGAAGAAAAGTGCCAGGAATATAAAGAGAAGAATCAAGCTAGAAGAATAGCATATATGCGTCAAATGGG
GCGTGAAAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT86729 NZ_CP024106:28902-28998 [Bacillus cytotoxicus]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin