Detailed information of TA system TA086728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 1952812..1953804
• Replicon: chromosome
• Accession: NZ_CP024104
• Organism: Bacillus cytotoxicus strain CH_23

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A7GPN6
• Locus tag: CG481_RS10110
• Protein ID: WP_012094283.1
• Coordinates: 1952812..1953552 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A7GPN7
• Locus tag: CG481_RS10115
• Protein ID: WP_012094285.1
• Coordinates: 1953676..1953804 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG481_RS10080	1948286..1949188	+	903	WP_012094278.1	ATPase	-
CG481_RS10085	1949235..1949541	-	307	Protein_1846	hypothetical protein	-
CG481_RS10090	1949736..1951040	+	1305	WP_012094279.1	DEAD/DEAH box helicase	-
CG481_RS10095	1951260..1951742	+	483	WP_012094281.1	MarR family winged helix-turn-helix transcriptional regulator	-
CG481_RS10100	1951880..1952617	+	738	WP_012094282.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CG481_RS10110	1952812..1953552	+	741	WP_012094283.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CG481_RS10115	1953676..1953804	+	129	WP_012094285.1	hypothetical protein	Antitoxin
CG481_RS10120	1953881..1954057	+	177	WP_012094286.1	hypothetical protein	-
CG481_RS10125	1954216..1954761	-	546	WP_012094287.1	GNAT family N-acetyltransferase	-
CG481_RS10130	1954802..1955683	-	882	WP_012094288.1	aminoglycoside 6-adenylyltransferase	-
CG481_RS10135	1955889..1957358	+	1470	WP_012094289.1	aminoacyl-histidine dipeptidase	-
CG481_RS10140	1957962..1958213	+	252	WP_012094290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28628.30 Da
• Isoelectric Point: 6.7302

>T86728 WP_012094283.1 NZ_CP024104:1952812-1953552 [Bacillus cytotoxicus]
MTVTSVRIGLFFLVIFFLFLIFFYWRNEERYEERKQLIRKTWYGLFITSVTMYFMMKGIDLTLWKHILMVVAMVVFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTLIQNVNPAAFGTMEWETDAEYARSLNAFLDTYAEKIGAK
IVVFVTEEELNTNFRGVRSQFSITVSPESIEQLNEQKAVQIENVGIIPAKIIKDVYIIMDGQKNNLQDRDFENVYNLTIH
HSYFCK

Nucleotide

• Length: 741 bp

>T86728 NZ_CP024104:1952812-1953552 [Bacillus cytotoxicus]
TTGACTGTCACTAGCGTTCGAATTGGCTTGTTTTTTCTTGTAATCTTTTTTTTATTTCTTATTTTCTTTTATTGGAGAAA
TGAAGAACGATACGAGGAAAGAAAACAACTTATTCGAAAGACGTGGTATGGTTTATTTATTACATCAGTCACAATGTATT
TCATGATGAAAGGAATTGATTTAACCCTCTGGAAACATATTTTAATGGTTGTTGCGATGGTTGTATTTGTTGATATTGCA
TTTATTTTAACGCCAAATATTTCGGAAATCTGGGGCGCAAAGTTTAGTGATATTGGTAAGACAGTTCAATCGATTAAACG
ATCGCTTATTGCTTCAAAAGCAAGAGGAGAAATATATACAACACTTATTCAAAATGTAAATCCAGCTGCATTTGGTACGA
TGGAATGGGAAACAGATGCTGAGTATGCACGCAGTTTAAATGCTTTTCTTGATACGTATGCTGAAAAAATAGGTGCGAAA
ATTGTAGTATTTGTCACAGAAGAGGAATTAAATACAAACTTCCGAGGTGTTCGATCTCAATTTAGTATTACAGTATCACC
CGAATCTATTGAACAACTGAATGAGCAAAAAGCAGTTCAAATTGAAAATGTTGGAATTATACCAGCGAAAATAATAAAAG
ACGTTTATATTATTATGGATGGACAAAAAAATAATTTGCAAGATCGTGACTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTACTTTTGTAAGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4984.43 Da
• Isoelectric Point: 3.9944

>AT86728 WP_012094285.1 NZ_CP024104:1953676-1953804 [Bacillus cytotoxicus]
VLKEYSNDFFKDEAEDTTDFSLIKGDTMQQVEELEEELKRRG

Nucleotide

• Length: 129 bp

>AT86728 NZ_CP024104:1953676-1953804 [Bacillus cytotoxicus]
GTGTTAAAAGAATATAGCAATGATTTCTTTAAAGACGAAGCTGAGGATACAACAGATTTTTCTTTAATAAAAGGAGATAC
AATGCAGCAAGTGGAAGAATTAGAAGAAGAATTGAAAAGAAGAGGATAA

Structures

Toxin

• AlphaFold DB: A7GPN6

Antitoxin

• AlphaFold DB: A7GPN7