Detailed information of TA system TA086725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 1952831..1953823
• Replicon: chromosome
• Accession: NZ_CP024101
• Organism: Bacillus cytotoxicus strain CH_25

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A7GPN6
• Locus tag: CG482_RS10095
• Protein ID: WP_012094283.1
• Coordinates: 1952831..1953571 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A7GPN7
• Locus tag: CG482_RS10100
• Protein ID: WP_012094285.1
• Coordinates: 1953695..1953823 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG482_RS10065	1948305..1949207	+	903	WP_012094278.1	ATPase	-
CG482_RS10070	1949254..1949560	-	307	Protein_1846	hypothetical protein	-
CG482_RS10075	1949755..1951059	+	1305	WP_012094279.1	DEAD/DEAH box helicase	-
CG482_RS10080	1951279..1951761	+	483	WP_012094281.1	MarR family winged helix-turn-helix transcriptional regulator	-
CG482_RS10085	1951899..1952636	+	738	WP_012094282.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CG482_RS10095	1952831..1953571	+	741	WP_012094283.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CG482_RS10100	1953695..1953823	+	129	WP_012094285.1	hypothetical protein	Antitoxin
CG482_RS10105	1953900..1954076	+	177	WP_012094286.1	hypothetical protein	-
CG482_RS10110	1954234..1954779	-	546	WP_012094287.1	GNAT family N-acetyltransferase	-
CG482_RS10115	1954820..1955701	-	882	WP_012094288.1	aminoglycoside 6-adenylyltransferase	-
CG482_RS10120	1955907..1957376	+	1470	WP_012094289.1	aminoacyl-histidine dipeptidase	-
CG482_RS10125	1957981..1958232	+	252	WP_012094290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28628.30 Da
• Isoelectric Point: 6.7302

>T86725 WP_012094283.1 NZ_CP024101:1952831-1953571 [Bacillus cytotoxicus]
MTVTSVRIGLFFLVIFFLFLIFFYWRNEERYEERKQLIRKTWYGLFITSVTMYFMMKGIDLTLWKHILMVVAMVVFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTLIQNVNPAAFGTMEWETDAEYARSLNAFLDTYAEKIGAK
IVVFVTEEELNTNFRGVRSQFSITVSPESIEQLNEQKAVQIENVGIIPAKIIKDVYIIMDGQKNNLQDRDFENVYNLTIH
HSYFCK

Nucleotide

• Length: 741 bp

>T86725 NZ_CP024101:1952831-1953571 [Bacillus cytotoxicus]
TTGACTGTCACTAGCGTTCGAATTGGCTTGTTTTTTCTTGTAATCTTTTTTTTATTTCTTATTTTCTTTTATTGGAGAAA
TGAAGAACGATACGAGGAAAGAAAACAACTTATTCGAAAGACGTGGTATGGTTTATTTATTACATCAGTCACAATGTATT
TCATGATGAAAGGAATTGATTTAACCCTCTGGAAACATATTTTAATGGTTGTTGCGATGGTTGTATTTGTTGATATTGCA
TTTATTTTAACGCCAAATATTTCGGAAATCTGGGGCGCAAAGTTTAGTGATATTGGTAAGACAGTTCAATCGATTAAACG
ATCGCTTATTGCTTCAAAAGCAAGAGGAGAAATATATACAACACTTATTCAAAATGTAAATCCAGCTGCATTTGGTACGA
TGGAATGGGAAACAGATGCTGAGTATGCACGCAGTTTAAATGCTTTTCTTGATACGTATGCTGAAAAAATAGGTGCGAAA
ATTGTAGTATTTGTCACAGAAGAGGAATTAAATACAAACTTCCGAGGTGTTCGATCTCAATTTAGTATTACAGTATCACC
CGAATCTATTGAACAACTGAATGAGCAAAAAGCAGTTCAAATTGAAAATGTTGGAATTATACCAGCGAAAATAATAAAAG
ACGTTTATATTATTATGGATGGACAAAAAAATAATTTGCAAGATCGTGACTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTACTTTTGTAAGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4984.43 Da
• Isoelectric Point: 3.9944

>AT86725 WP_012094285.1 NZ_CP024101:1953695-1953823 [Bacillus cytotoxicus]
VLKEYSNDFFKDEAEDTTDFSLIKGDTMQQVEELEEELKRRG

Nucleotide

• Length: 129 bp

>AT86725 NZ_CP024101:1953695-1953823 [Bacillus cytotoxicus]
GTGTTAAAAGAATATAGCAATGATTTCTTTAAAGACGAAGCTGAGGATACAACAGATTTTTCTTTAATAAAAGGAGATAC
AATGCAGCAAGTGGAAGAATTAGAAGAAGAATTGAAAAGAAGAGGATAA

Structures

Toxin

• AlphaFold DB: A7GPN6

Antitoxin

• AlphaFold DB: A7GPN7