Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 251941..252583 Replicon chromosome
Accession NZ_CP024101
Organism Bacillus cytotoxicus strain CH_25

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag CG482_RS01450 Protein ID WP_000635963.1
Coordinates 252233..252583 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8PTJ6
Locus tag CG482_RS01445 Protein ID WP_000004571.1
Coordinates 251941..252228 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CG482_RS01425 248320..248880 - 561 WP_048721301.1 rhomboid family intramembrane serine protease -
CG482_RS01430 248971..249330 + 360 WP_011983363.1 holo-ACP synthase -
CG482_RS01435 249486..250436 + 951 WP_041810183.1 outer membrane lipoprotein carrier protein LolA -
CG482_RS01440 250558..251727 + 1170 WP_011983365.1 alanine racemase -
CG482_RS01445 251941..252228 + 288 WP_000004571.1 antitoxin EndoAI Antitoxin
CG482_RS01450 252233..252583 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CG482_RS01455 252648..254816 + 2169 WP_048721313.1 RNA-binding transcriptional accessory protein -
CG482_RS01460 254890..255024 - 135 WP_087094358.1 cortex morphogenetic protein CmpA -
CG482_RS01465 255411..255875 + 465 WP_011983367.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T86724 WP_000635963.1 NZ_CP024101:252233-252583 [Bacillus cytotoxicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T86724 NZ_CP024101:252233-252583 [Bacillus cytotoxicus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCAGCGATTACTGCACAGATTCAGAAAG
CAAAATTGCCCACGCATGTGGAGATTGATGCGAAGAAGTACGGATTTGAGAGGGATTCTGTGATCTTGCTCGAGCAGATT
CGAACAATTGATAAGCAACGCTTAACGGACAAAATCACACATTTAGATGAAGTGATGATGATTCGTGTAGATGAGGCTTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10748.30 Da        Isoelectric Point: 8.9605

>AT86724 WP_000004571.1 NZ_CP024101:251941-252228 [Bacillus cytotoxicus]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT86724 NZ_CP024101:251941-252228 [Bacillus cytotoxicus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAGTGGTGACGGAATTGGACGGGATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAAAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB R8PTJ6

References